Transcatheter therapies are emerging for functional mitral regurgitation (FMR) treatment, however there is lack of pathological models for their preclinical assessment. We investigated the applicability of deer hearts for this purpose. 8 whole deer hearts were housed in a pulsatile flow bench. At baseline, all mitral valves featured normal coaptation. The pathological state was induced by 60-minutes intraventricular constant pressurization. It caused mitral annulus dilation (antero-posterior diameter increase from 31.8 ± 5.6 mm to 39.5 ± 4.9 mm, p = 0.001), leaflets tethering (maximal tenting height increase from 7.3 ± 2.5 mm to 12.7 ± 3.4 mm, p < 0.001) and left ventricular diameter increase (from 67.8 ± 7.5 mm to 79.4 ± 6.5 mm, p = 0.004). These geometrical reconfigurations led to restricted mitral valve leaflets motion and leaflet coaptation loss. Preliminary feasibility assessment of two FMR treatments was performed in the developed model. Deer hearts showed ability to dilate under constant pressurization and have potential to be used for realistic preclinical research of novel FMR therapies.

Ex Vivo Model of Functional Mitral Regurgitation Using Deer Hearts / M. Jaworek, A. Mangini, E. Maroncelli, F. Lucherini, R. Rosa, E. Salurso, E. Votta, C. Antona, G.B. Fiore, R. Vismara. - In: JOURNAL OF CARDIOVASCULAR TRANSLATIONAL RESEARCH. - ISSN 1937-5387. - 14:3(2021), pp. 513-524. [10.1007/s12265-020-10071-y]

Ex Vivo Model of Functional Mitral Regurgitation Using Deer Hearts

C. Antona;
2021

Abstract

Transcatheter therapies are emerging for functional mitral regurgitation (FMR) treatment, however there is lack of pathological models for their preclinical assessment. We investigated the applicability of deer hearts for this purpose. 8 whole deer hearts were housed in a pulsatile flow bench. At baseline, all mitral valves featured normal coaptation. The pathological state was induced by 60-minutes intraventricular constant pressurization. It caused mitral annulus dilation (antero-posterior diameter increase from 31.8 ± 5.6 mm to 39.5 ± 4.9 mm, p = 0.001), leaflets tethering (maximal tenting height increase from 7.3 ± 2.5 mm to 12.7 ± 3.4 mm, p < 0.001) and left ventricular diameter increase (from 67.8 ± 7.5 mm to 79.4 ± 6.5 mm, p = 0.004). These geometrical reconfigurations led to restricted mitral valve leaflets motion and leaflet coaptation loss. Preliminary feasibility assessment of two FMR treatments was performed in the developed model. Deer hearts showed ability to dilate under constant pressurization and have potential to be used for realistic preclinical research of novel FMR therapies.
Atrial mitral regurgitation; Beating heart model; Ex vivo; Functional mitral regurgitation; Heart valve pathological model; Ischemic mitral regurgitation; Mitral valve; Preclinical research; Animals; Deer; Disease Models, Animal; Echocardiography, Three-Dimensional; Fiber Optic Technology; Isolated Heart Preparation; Mitral Valve; Mitral Valve Insufficiency; Ventricular Function, Left; Hemodynamics
Settore MED/23 - Chirurgia Cardiaca
21-set-2020
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/904590
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