Mutations in the intracellular juxtamembrane domain of the c-kit gene in 32 dogs with different grades of histologically confirmed mastocytoma were studied. Transcript RNAs extracted from neoplastic tissue surgically collected from dogs of different breeds and from a negative control were reverse transcribed into complementary DNA and amplified by polymerase chain reaction. The region corresponding to the c-kit juxtamembrane domain was sequenced and compared with GenBank sequences. Two different types of mutations were identified within exon 11: a previously underscribed single-nucleotide substitution and a 6-bp deletion. The c-kit juxtamembrane domain sequences of all dogs were grouped in 3 clusters. No mutations were detected in tissues constitutively expressing c-kit (cerebellum and spleen), obtained from dogs not affected by mastocytoma (controls). All the substitutions were found in dogs bearing grade I or II mast cell tumors; the deletion was detected in 1 dog with grade II mastocytoma.
A study of mutations in the c-kit gene of 32 dogs with mastocytoma / F. Riva, S. Brizzola, D. Stefanello, S. Crema, L. Turin. - In: JOURNAL OF VETERINARY DIAGNOSTIC INVESTIGATION. - ISSN 1040-6387. - 17:4(2005), pp. 385-388.
|Titolo:||A study of mutations in the c-kit gene of 32 dogs with mastocytoma|
RIVA, FEDERICA (Primo)
TURIN, LAURETTA (Ultimo)
|Parole Chiave:||c-kit; Canine; Dogs; KIT receptor; Mastocytoma; Mutation; Oncogene|
|Settore Scientifico Disciplinare:||Settore VET/05 - Malattie Infettive degli Animali Domestici|
|Data di pubblicazione:||2005|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1177/104063870501700416|
|Appare nelle tipologie:||01 - Articolo su periodico|