Monocytes/macrophages play critical roles in HIV transmission, viral spread (early in infection), and as a reservoir of virus throughout infection. In the current research area in HIV, there has been a recent resurgence of interest in the biology of monocyte subsets and macrophages and their role in HIV pathogenesis, and as long-lived HIV reservoir. Thus, sensitive and specific techniques are needed to measure the impact of these cells in the establishment of the “hard-core” reservoir, and in their capacity to cause a low-level virus production during cART. Here, a protocol is presented for cell culture and HIV-1 infection of granulocyte–macrophage colony-stimulating factor (GM-CSF) differentiated human monocyte-derived macrophages.
Human Monocyte-Derived Macrophages (MDM): Model 1 (GM-CSF) / C. Alteri, L. Piermatteo, F.C. Silberstein, V. Svicher, C.F. Perno (METHODS IN MOLECULAR BIOLOGY). - In: HIV Reservoirs / [a cura di] G. Poli, E. Vicenzi, F. Romerio. - [s.l] : Humana Press Inc., 2022. - ISBN 978-1-0716-1870-7. - pp. 91-96 [10.1007/978-1-0716-1871-4_8]
Human Monocyte-Derived Macrophages (MDM): Model 1 (GM-CSF)
C. AlteriPrimo
;C.F. Perno
2022
Abstract
Monocytes/macrophages play critical roles in HIV transmission, viral spread (early in infection), and as a reservoir of virus throughout infection. In the current research area in HIV, there has been a recent resurgence of interest in the biology of monocyte subsets and macrophages and their role in HIV pathogenesis, and as long-lived HIV reservoir. Thus, sensitive and specific techniques are needed to measure the impact of these cells in the establishment of the “hard-core” reservoir, and in their capacity to cause a low-level virus production during cART. Here, a protocol is presented for cell culture and HIV-1 infection of granulocyte–macrophage colony-stimulating factor (GM-CSF) differentiated human monocyte-derived macrophages.File | Dimensione | Formato | |
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