Equine bone blocks have osteogenic effects promoting bone regeneration with biocompatibility and osteoconductivity capacity. Human dental pulp stem cells (hDPSCs) can differentiate into osteoblasts enhancing biomineralization with such scaffolds. Melatonin is able to improve bone health and mediate bone formation. Collagenated equine bone blocks were coated with ammoniafunctionalized graphene-oxide (G-N) at two different concentrations (2 ug/mL, G-N2; and 10 ug/mL, G-N10). The homogeneity of G-N coating was checked by Raman spectroscopy, whereas thermogravimetric analysis (TGA) allowed us to quantify the amount of G-N deposited on the blocks. The aim of this study was to investigate in vitro the effect of G-N-coated collagenated equine bone blocks on the proliferation and differentiation of hDPSCs with the addition of a melatonin. This evaluation was determined after 7, 14, and 21 days of culture by the expression of specific microRNAs, RUNX2 and SMAD5 gene expression, osteocalcin levels, and histological analysis. The results showed that equine blocks G-N2 and G-N10 and melatonin gave an optimal cell adhesion as shown by histological analysis, and an increase in the hDPSCs osteogenic potential as confirmed by microRNA and gene expression with an increase in osteocalcin levels. This study suggests that equine bone blocks coated with G-N2 and G-N10 and melatonin promote the osteogenic process.

Human dental pulp stem cell osteogenic differentiation seeded on equine bone block with graphene and melatonin / R. Mancinelli, E.S. Di Filippo, M. Tumedei, M. Marrone, A. Fontana, V. Ettorre, S. Giordani, M. Baldrighi, G. Iezzi, A. Piattelli, S. Fulle. - In: APPLIED SCIENCES. - ISSN 2076-3417. - 11:7(2021), pp. 3218.1-3218.19. [10.3390/app11073218]

Human dental pulp stem cell osteogenic differentiation seeded on equine bone block with graphene and melatonin

M. Tumedei;
2021

Abstract

Equine bone blocks have osteogenic effects promoting bone regeneration with biocompatibility and osteoconductivity capacity. Human dental pulp stem cells (hDPSCs) can differentiate into osteoblasts enhancing biomineralization with such scaffolds. Melatonin is able to improve bone health and mediate bone formation. Collagenated equine bone blocks were coated with ammoniafunctionalized graphene-oxide (G-N) at two different concentrations (2 ug/mL, G-N2; and 10 ug/mL, G-N10). The homogeneity of G-N coating was checked by Raman spectroscopy, whereas thermogravimetric analysis (TGA) allowed us to quantify the amount of G-N deposited on the blocks. The aim of this study was to investigate in vitro the effect of G-N-coated collagenated equine bone blocks on the proliferation and differentiation of hDPSCs with the addition of a melatonin. This evaluation was determined after 7, 14, and 21 days of culture by the expression of specific microRNAs, RUNX2 and SMAD5 gene expression, osteocalcin levels, and histological analysis. The results showed that equine blocks G-N2 and G-N10 and melatonin gave an optimal cell adhesion as shown by histological analysis, and an increase in the hDPSCs osteogenic potential as confirmed by microRNA and gene expression with an increase in osteocalcin levels. This study suggests that equine bone blocks coated with G-N2 and G-N10 and melatonin promote the osteogenic process.
Bone regeneration; Equine bone block; Graphene-oxide; HDPSC; Melatonin; Osteogenesis
Settore MED/50 - Scienze Tecniche Mediche Applicate
Settore MED/28 - Malattie Odontostomatologiche
Article (author)
File in questo prodotto:
File Dimensione Formato  
GRAFENEapplsci-11-03218.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 3.32 MB
Formato Adobe PDF
3.32 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/896554
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 2
  • ???jsp.display-item.citation.isi??? 2
social impact