Moyamoya arteriopathy (MA) is a rare cerebrovascular disorder characterized by is-chemic/hemorrhagic strokes. The pathophysiology is unknown. A deregulation of vasculogenic/ angiogenic/inflammatory pathways has been hypothesized as a possible pathophysiological mechanism. Since lipids are implicated in modulating neo-vascularization/angiogenesis and inflammation, their deregulation is potentially involved in MA. Our aim is to evaluate angiogenic/vasculogenic/ inflammatory proteins and lipid profile in plasma of MA patients and control subjects (healthy donors HD or subjects with atherosclerotic cerebrovascular disease ACVD). Angiogenic and inflammatory protein levels were measured by ELISA and a complete lipidomic analysis was performed on plasma by mass spectrometry. ELISA showed a significant decrease for MMP-9 released in plasma of MA. The untargeted lipidomic analysis showed a cumulative depletion of lipid asset in plasma of MA as compared to HD. Specifically, a decrease in membrane complex glycosphingolipids peripherally circulating in MA plasma with respect to HD was observed, likely suggestive of cerebral cellular recruitment. The quantitative targeted approach demonstrated an increase in free sphingoid bases, likely associated with a deregulated angiogenesis. Our findings indicate that lipid signature could play a central role in MA and that a detailed biomarker profile may contribute to untangle the complex, and still obscure, pathogenesis of MA.

Plasma Lipid Profiling Contributes to Untangle the Complexity of Moyamoya Arteriopathy / M.V. Dei Cas, T. Carrozzini, G. Pollaci, A. Potenza, S. Nava, I. Canavero, F. Tinelli, G. Gorla, I. Gaspare Vetrano, F. Acerbi, P. Ferroli, E. Francesca Maria Ciceri, S. Esposito, V. Saletti, E. Ciusani, A. Zulueta Morales, R.C. Paroni, E.A. Parati, R. Ghidoni, A. Bersano, L. Gatti. - In: INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES. - ISSN 1422-0067. - 22:24(2021 Dec 02), pp. 13410.1-13410.16. [10.3390/ijms222413410]

Plasma Lipid Profiling Contributes to Untangle the Complexity of Moyamoya Arteriopathy

M.V. DEI CAS
Primo
;
A. ZULUETA MORALES;R.C. Paroni;R. Ghidoni;
2021

Abstract

Moyamoya arteriopathy (MA) is a rare cerebrovascular disorder characterized by is-chemic/hemorrhagic strokes. The pathophysiology is unknown. A deregulation of vasculogenic/ angiogenic/inflammatory pathways has been hypothesized as a possible pathophysiological mechanism. Since lipids are implicated in modulating neo-vascularization/angiogenesis and inflammation, their deregulation is potentially involved in MA. Our aim is to evaluate angiogenic/vasculogenic/ inflammatory proteins and lipid profile in plasma of MA patients and control subjects (healthy donors HD or subjects with atherosclerotic cerebrovascular disease ACVD). Angiogenic and inflammatory protein levels were measured by ELISA and a complete lipidomic analysis was performed on plasma by mass spectrometry. ELISA showed a significant decrease for MMP-9 released in plasma of MA. The untargeted lipidomic analysis showed a cumulative depletion of lipid asset in plasma of MA as compared to HD. Specifically, a decrease in membrane complex glycosphingolipids peripherally circulating in MA plasma with respect to HD was observed, likely suggestive of cerebral cellular recruitment. The quantitative targeted approach demonstrated an increase in free sphingoid bases, likely associated with a deregulated angiogenesis. Our findings indicate that lipid signature could play a central role in MA and that a detailed biomarker profile may contribute to untangle the complex, and still obscure, pathogenesis of MA.
Angiogenesis; Glycosphingolipids; Inflammation; Lipidomics; MMP-9; Moyamoya arteriopathy; RNF213; Sphingosine; Vasculogenesis;
Settore BIO/10 - Biochimica
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
Settore MED/26 - Neurologia
Settore MED/27 - Neurochirurgia
Article (author)
File in questo prodotto:
File Dimensione Formato  
Plasma Lipid Profiling Contributes to Untangle the Complexity.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 2.42 MB
Formato Adobe PDF
2.42 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/891279
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 3
  • ???jsp.display-item.citation.isi??? 3
social impact