In vitro maturation (IVM) has been applied in numerous different contexts and strategies in humans and animals, but in both cases it represents a challenge still far from being overcome. Despite the large dataset produced over the last two decades on the mechanisms that govern antral follicular development and oocyte metabolism and differentiation, IVM outcomes are still unsatisfactory. This review specifically focuses on data concerning the potential consequences of using supraphysiological levels of FSH during IVM, as well as on the regulation of oocyte chromatin dynamics and its utility as a potential marker of oocyte developmental competence. Taken together, the data revisited herein indicate that a significant improvement in IVM efficacy may be provided by the integration of pre-OPU patient-specific protocols preparing the oocyte population for IVM and more physiological culture systems mimicking more precisely the follicular environment that would be experienced by the recovered oocytes until completion of metaphase II.

Physiological parameters related to oocyte nuclear differentiation for the improvement of IVM/IVF outcomes in women and cattle / J. BuratiniA, A. Caroline Silva Soares, R. Garcia Barros, T. Tino Dellaqua, V. Lodde, F. Franciosi, M. Dal Canto, M. Mignini Renzini, A.M. Luciano. - In: REPRODUCTION FERTILITY AND DEVELOPMENT. - ISSN 1031-3613. - 34:2(2021), pp. 27-35. [doi:10.1071/RD21278]

Physiological parameters related to oocyte nuclear differentiation for the improvement of IVM/IVF outcomes in women and cattle

R. GARCIA BARROS;V. Lodde;F. Franciosi;A.M. Luciano
Ultimo
2021

Abstract

In vitro maturation (IVM) has been applied in numerous different contexts and strategies in humans and animals, but in both cases it represents a challenge still far from being overcome. Despite the large dataset produced over the last two decades on the mechanisms that govern antral follicular development and oocyte metabolism and differentiation, IVM outcomes are still unsatisfactory. This review specifically focuses on data concerning the potential consequences of using supraphysiological levels of FSH during IVM, as well as on the regulation of oocyte chromatin dynamics and its utility as a potential marker of oocyte developmental competence. Taken together, the data revisited herein indicate that a significant improvement in IVM efficacy may be provided by the integration of pre-OPU patient-specific protocols preparing the oocyte population for IVM and more physiological culture systems mimicking more precisely the follicular environment that would be experienced by the recovered oocytes until completion of metaphase II.
Settore VET/01 - Anatomia degli Animali Domestici
Settore VET/02 - Fisiologia Veterinaria
Settore VET/10 - Clinica Ostetrica e Ginecologia Veterinaria
Settore MED/13 - Endocrinologia
Settore MED/40 - Ginecologia e Ostetricia
2021
8-nov-2021
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/891200
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