Protein-protein interactions (PPIs) contribute to regulate many aspects of cell physiology and metabolism. Protein domains involved in PPIs are important building blocks for engineering genetic circuits through synthetic biology. These domains can be obtained from known proteins and rationally engineered to produce orthogonal scaffolds, or computationally designed de novo thanks to recent advances in structural biology and molecular dynamics prediction. Such circuits based on PPIs (or protein circuits) appear of particular interest, as they can directly affect transcriptional outputs, as well as induce behavioral/adaptational changes in cell metabolism, without the need for further protein synthesis. This last example was highlighted in recent works to enable the production of fast-responding circuits which can be exploited for biosensing and diagnostics. Notably, PPIs can also be engineered to develop new drugs able to bind specific intra- and extra-cellular targets. In this review, we summarize recent findings in the field of protein circuit design, with particular focus on the use of peptides as scaffolds to engineer these circuits.

Synthetic Protein Circuits and Devices Based on Reversible Protein-Protein Interactions: An Overview / S. Rosa, C. Bertaso, P. Pesaresi, S. Masiero, A. Tagliani. - In: LIFE. - ISSN 2075-1729. - 11:11(2021 Nov 03), pp. 1171.1-1171.10. [10.3390/life11111171]

Synthetic Protein Circuits and Devices Based on Reversible Protein-Protein Interactions: An Overview

S. Rosa
Primo
;
P. Pesaresi;S. Masiero
Penultimo
;
A. Tagliani
Ultimo
2021

Abstract

Protein-protein interactions (PPIs) contribute to regulate many aspects of cell physiology and metabolism. Protein domains involved in PPIs are important building blocks for engineering genetic circuits through synthetic biology. These domains can be obtained from known proteins and rationally engineered to produce orthogonal scaffolds, or computationally designed de novo thanks to recent advances in structural biology and molecular dynamics prediction. Such circuits based on PPIs (or protein circuits) appear of particular interest, as they can directly affect transcriptional outputs, as well as induce behavioral/adaptational changes in cell metabolism, without the need for further protein synthesis. This last example was highlighted in recent works to enable the production of fast-responding circuits which can be exploited for biosensing and diagnostics. Notably, PPIs can also be engineered to develop new drugs able to bind specific intra- and extra-cellular targets. In this review, we summarize recent findings in the field of protein circuit design, with particular focus on the use of peptides as scaffolds to engineer these circuits.
synthetic biology; protein circuits; protein-protein interactions; combinatorial libraries; peptides;
Settore BIO/11 - Biologia Molecolare
H20_RIA19PPESA_01 - Novel Pesticides for a Sustainable Agriculture (NoPest) - PESARESI, PAOLO - H20_RIA - Horizon 2020_Research & Innovation Action/Innovation Action - 2019
PRIN201719SMASI_01 - Small RNAs and peptides for controlling diseases and development in horticultural plants - MASIERO, SIMONA - PRIN2017 - PRIN bando 2017 - 2019
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/888938
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