Cell wall modifications are of pivotal importance during plant development. Among cell wall components, xyloglucans are the major hemicellulose polysaccharide in primary cell walls of dicots and non graminaceous monocots. They can connect the cellulose microfibril surface to affect cell wall mechanical properties. Changes in xyloglucan structure are known to play an important role regulating cell growth. Therefore, the degradation of xyloglucan is an important modification that alters the cell wall. The α-XYLOSIDASE1 (XYL1) gene encodes the only α-xylosidase acting on xyloglucans in Arabidopsis thaliana. Here, we show that mutation of XYL1 strongly influences seed size, seed germination, and fruit elongation. We found that the expression of XYL1 is directly regulated in developing seeds and fruit by the MADS-box transcription factor SEEDSTICK (STK). We demonstrate that XYL1 complements the stk smaller seed phenotype. Finally, by Atomic Force Microscopy (AFM), we investigate the role of XYL1 activity in maintaining cell stiffness and growth, confirming the importance of cell wall modulation in shaping organs.

Cell wall modifications by α-XYLOSIDASE1 are required for control of seed and fruit size in Arabidopsis / M. Di Marzo, V.E. Viana, C. Banfi, V. Cassina, R. Corti, H. Herrera-Ubaldo, N. Babolin, A. Guazzotti, E. Kiegle, V. Gregis, S. de Folter, J. Sampedro, F. Mantegazza, L. Colombo, I. Ezquer. - In: JOURNAL OF EXPERIMENTAL BOTANY. - ISSN 0022-0957. - 73:5(2022 Mar 02), pp. 1499-1515. [10.1093/jxb/erab514]

Cell wall modifications by α-XYLOSIDASE1 are required for control of seed and fruit size in Arabidopsis

M. Di Marzo
Primo
;
C. Banfi;A. Guazzotti;E. Kiegle;V. Gregis;L. Colombo
Penultimo
;
I. Ezquer
Ultimo
2022

Abstract

Cell wall modifications are of pivotal importance during plant development. Among cell wall components, xyloglucans are the major hemicellulose polysaccharide in primary cell walls of dicots and non graminaceous monocots. They can connect the cellulose microfibril surface to affect cell wall mechanical properties. Changes in xyloglucan structure are known to play an important role regulating cell growth. Therefore, the degradation of xyloglucan is an important modification that alters the cell wall. The α-XYLOSIDASE1 (XYL1) gene encodes the only α-xylosidase acting on xyloglucans in Arabidopsis thaliana. Here, we show that mutation of XYL1 strongly influences seed size, seed germination, and fruit elongation. We found that the expression of XYL1 is directly regulated in developing seeds and fruit by the MADS-box transcription factor SEEDSTICK (STK). We demonstrate that XYL1 complements the stk smaller seed phenotype. Finally, by Atomic Force Microscopy (AFM), we investigate the role of XYL1 activity in maintaining cell stiffness and growth, confirming the importance of cell wall modulation in shaping organs.
Cell wall; MADS-box; fruit growth; seed size; transcription factor; xyloglucan
Settore BIO/01 - Botanica Generale
Settore BIO/04 - Fisiologia Vegetale
Settore BIO/18 - Genetica
Settore AGR/07 - Genetica Agraria
25-nov-2021
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/887666
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