Aim of the study: The purpose of this study is to evaluate the sensitivity and specificity of free circulating DNA (CFDNA) as a potential tumour marker in patients suffering from colorectal cancer (CRC). Materials and methods: The quantification of plasma DNA was determined through the use of real-time quantitative polymerase chain reaction (PCR) amplification of the RNAse P gene. We studied patients undergoing surgery for primary CRC, at different stages of disease and a cohort of comparable healthy donors. Data were statistically analysed using Student's t-test. Results: The mean DNA index of the tumour samples was 4771 ng/ml, about 50 times higher in comparison with healthy donors (mean value 0.85 ng/ml). In contrast the carcinoembryonic antigen (CEA) value of these patients was altered in only 47% of cases. Conclusion: This study shows that circulating free DNA levels in plasma of patients with CRC are significantly higher compared to healthy subjects, and suggest a new, non-invasive, approach for colon cancer detection and to identify high risk individuals for CRC screening.

Free circulating DNA as possible tumour marker in colorectal cancer / L. Boni, E. Cassinotti, M. Canziani, G. Dionigi, F. Rovera, D. R.. - In: SURGICAL ONCOLOGY. - ISSN 0960-7404. - 16:suppl. 1(2007), pp. S29-S31. [10.1016/j.suronc.2007.10.004]

Free circulating DNA as possible tumour marker in colorectal cancer

L. Boni
Primo
;
G. Dionigi;
2007

Abstract

Aim of the study: The purpose of this study is to evaluate the sensitivity and specificity of free circulating DNA (CFDNA) as a potential tumour marker in patients suffering from colorectal cancer (CRC). Materials and methods: The quantification of plasma DNA was determined through the use of real-time quantitative polymerase chain reaction (PCR) amplification of the RNAse P gene. We studied patients undergoing surgery for primary CRC, at different stages of disease and a cohort of comparable healthy donors. Data were statistically analysed using Student's t-test. Results: The mean DNA index of the tumour samples was 4771 ng/ml, about 50 times higher in comparison with healthy donors (mean value 0.85 ng/ml). In contrast the carcinoembryonic antigen (CEA) value of these patients was altered in only 47% of cases. Conclusion: This study shows that circulating free DNA levels in plasma of patients with CRC are significantly higher compared to healthy subjects, and suggest a new, non-invasive, approach for colon cancer detection and to identify high risk individuals for CRC screening.
Colorectal cancer; Free circulating DNA.; Marker
Settore MED/18 - Chirurgia Generale
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/883897
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