Twelve prepubertal (35-45-day-old) and 13 adult (3-6-year-old) goats were stimulated and COCs were collected by OPU and matured in vitro. After oocyte in vitro fertilization and culture, blastocysts were vitrified/warmed and cultured in vitro to assess blastocoelic re-expansion and gene expression. Prepubertals produced more COCs (p < 0.01) than adults. Degenerated COCs number (p < 0.01) was higher in prepubertals than adults and lower cleavage (p < 0.01) and blastocyst rates (p < 0.05) were evidenced in prepubertal compared to adult fertilized oocytes. Prepubertal vitrified/warmed blastocysts present a lower viability (p < 0.05) than adults. No significant differences were found in the expression of Cyclin B1, E-caderin, Na/K-ATPase, HSP90 and Aquaporin 3 genes. In conclusion our results evidenced a low development and cryotolerance of prepubertal goat embryos. However, the quality of viable prepubertal embryos after vitrification do not differ from the adult counterpart.
Cryotolerance and gene expression of in vitro produced prepubertal goat blastocysts / G. Leoni, S. Succu, V. Satta, L. Bogliolo, D. Bebbere, A. Spezzigu, M. Madeddu, A. Tedde, F. Berlinguer, S. Ledda, S. Naitana. - In: COMPTES RENDUS DE L'ACADÉMIE BULGARE DES SCIENCES. - ISSN 1310-1331. - 62:3(2009), pp. 367-372. (Intervento presentato al 1. convegno Conference of the Balkan-Network-for-Animal-Reproduction-Biotechnology : February, 18th - 19th tenutosi a Sofia nel 2009).
Cryotolerance and gene expression of in vitro produced prepubertal goat blastocysts
M. Madeddu;
2009
Abstract
Twelve prepubertal (35-45-day-old) and 13 adult (3-6-year-old) goats were stimulated and COCs were collected by OPU and matured in vitro. After oocyte in vitro fertilization and culture, blastocysts were vitrified/warmed and cultured in vitro to assess blastocoelic re-expansion and gene expression. Prepubertals produced more COCs (p < 0.01) than adults. Degenerated COCs number (p < 0.01) was higher in prepubertals than adults and lower cleavage (p < 0.01) and blastocyst rates (p < 0.05) were evidenced in prepubertal compared to adult fertilized oocytes. Prepubertal vitrified/warmed blastocysts present a lower viability (p < 0.05) than adults. No significant differences were found in the expression of Cyclin B1, E-caderin, Na/K-ATPase, HSP90 and Aquaporin 3 genes. In conclusion our results evidenced a low development and cryotolerance of prepubertal goat embryos. However, the quality of viable prepubertal embryos after vitrification do not differ from the adult counterpart.
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