N-methyl-d-aspartate receptor-mediated ( spikes can be causally linked to the induction of synaptic long-term potentiation (LTP) in hippocampal and cortical pyramidal cells. However, it is unclear if they regulate plasticity at a local or global scale in the dendritic tree. Here, we used dendritic patch-clamp recordings and calcium imaging to investigate the integrative properties of single dendrites of hippocampal CA3 cells. We show that local hyperpolarization of a single dendritic segment prevents NMDA spikes, their associated calcium transients, as well as LTP in a branch-specific manner. This result provides direct, causal evidence that the single dendritic branch can operate as a functional unit in regulating CA3 pyramidal cell plasticity.

Dendritic Branch-constrained N-Methyl-d-Aspartate Receptor-mediated Spikes Drive Synaptic Plasticity in Hippocampal CA3 Pyramidal Cells / F. Brandalise, S. Carta, R. Leone, F. Helmchen, A. Holtmaat, U. Gerber. - In: NEUROSCIENCE. - ISSN 0306-4522. - (2021). [Epub ahead of print] [10.1016/j.neuroscience.2021.10.002]

Dendritic Branch-constrained N-Methyl-d-Aspartate Receptor-mediated Spikes Drive Synaptic Plasticity in Hippocampal CA3 Pyramidal Cells

F. Brandalise;R. Leone;
2021

Abstract

N-methyl-d-aspartate receptor-mediated ( spikes can be causally linked to the induction of synaptic long-term potentiation (LTP) in hippocampal and cortical pyramidal cells. However, it is unclear if they regulate plasticity at a local or global scale in the dendritic tree. Here, we used dendritic patch-clamp recordings and calcium imaging to investigate the integrative properties of single dendrites of hippocampal CA3 cells. We show that local hyperpolarization of a single dendritic segment prevents NMDA spikes, their associated calcium transients, as well as LTP in a branch-specific manner. This result provides direct, causal evidence that the single dendritic branch can operate as a functional unit in regulating CA3 pyramidal cell plasticity.
CA3; LTP; dendrites; dendritic spike; hippocampus; plasticity
Settore BIO/09 - Fisiologia
9-ott-2021
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/878876
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