Coding and long non-coding RNA (lncRNA) metabolism is now revealing its crucial role in Amyotrophic Lateral Sclerosis (ALS) pathogenesis. In this work, we present a dataset obtained via Illumina RNA-seq analysis on Peripheral Blood Mononuclear Cells (PBMCs) from sporadic and mutated ALS patients (mutations in FUS, TARDBP, SOD1 and VCP genes) and healthy controls. This dataset allows the whole-transcriptome characterization of PBMCs content, both in terms of coding and non-coding RNAs, in order to compare the disease state to the healthy controls, both for sporadic patients and for mutated patients. Our dataset is a starting point for the omni-comprehensive analysis of coding and lncRNAs, from an easy to withdraw, manage and store tissue that shows to be a suitable model for RNA profiling in ALS.

RNA-seq profiling in peripheral blood mononuclear cells of amyotrophic lateral sclerosis patients and controls / S. Zucca, S. Gagliardi, C. Pandini, L. Diamanti, M. Bordoni, D. Sproviero, M. Arigoni, M. Olivero, O. Pansarasa, M. Ceroni, R. Calogero, C. Cereda. - In: SCIENTIFIC DATA. - ISSN 2052-4463. - 6:1(2019), pp. 190006.1-190006.8. [10.1038/sdata.2019.6]

RNA-seq profiling in peripheral blood mononuclear cells of amyotrophic lateral sclerosis patients and controls

C. Pandini;
2019

Abstract

Coding and long non-coding RNA (lncRNA) metabolism is now revealing its crucial role in Amyotrophic Lateral Sclerosis (ALS) pathogenesis. In this work, we present a dataset obtained via Illumina RNA-seq analysis on Peripheral Blood Mononuclear Cells (PBMCs) from sporadic and mutated ALS patients (mutations in FUS, TARDBP, SOD1 and VCP genes) and healthy controls. This dataset allows the whole-transcriptome characterization of PBMCs content, both in terms of coding and non-coding RNAs, in order to compare the disease state to the healthy controls, both for sporadic patients and for mutated patients. Our dataset is a starting point for the omni-comprehensive analysis of coding and lncRNAs, from an easy to withdraw, manage and store tissue that shows to be a suitable model for RNA profiling in ALS.
Amyotrophic Lateral Sclerosis; DNA-Binding Proteins; Humans; Leukocytes, Mononuclear; Mutation; RNA, Long Noncoding; RNA-Binding Protein FUS; Superoxide Dismutase-1; Valosin Containing Protein; Gene Expression Profiling
Settore BIO/11 - Biologia Molecolare
Settore BIO/13 - Biologia Applicata
Settore MED/04 - Patologia Generale
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/878439
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