Molecular imaging techniques are essential tools for better investigating biological processes and detecting disease biomarkers with improvement of both diagnosis and therapy monitoring. Often, a single imaging technique is not sufficient to obtain comprehensive information at different levels. Multimodal diagnostic probes are key tools to enable imaging across multiple scales. The direct registration of in vivo imaging markers with ex vivo imaging at the cellular level with a single probe is still challenging. Fluorinated (19F) probes have been increasingly showing promising potentialities for in vivo cell tracking by 19F-MRI. Here we present the unique features of a bioorthogonal 19F-probe that enables direct signal correlation of MRI with Raman imaging. In particular, we reveal the ability of PERFECTA, a superfluorinated molecule, to exhibit a remarkable intense Raman signal distinct from cell and tissue fingerprints. Therefore, PERFECTA combines in a single molecule excellent characteristics for both macroscopic in vivo 19F-MRI, across the whole body, and microscopic imaging at tissue and cellular levels by Raman imaging.
A bioorthogonal probe for multiscale imaging by 19F-MRI and Raman Microscopy: From whole body to single cells / C. Chirizzi, C. Morasso, A.A. Caldarone, M. Tommasini, F. Corsi, L. Chaabane, R. Vanna, F.B. Bombelli, P. Metrangolo. - In: JOURNAL OF THE AMERICAN CHEMICAL SOCIETY. - ISSN 0002-7863. - 143:31(2021 Aug 11), pp. 12253-12260. [10.1021/jacs.1c05250]
|Titolo:||A bioorthogonal probe for multiscale imaging by 19F-MRI and Raman Microscopy: From whole body to single cells|
|Settore Scientifico Disciplinare:||Settore BIO/11 - Biologia Molecolare|
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
|Data di pubblicazione:||11-ago-2021|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1021/jacs.1c05250|
|Appare nelle tipologie:||01 - Articolo su periodico|