Interferons (IFNs) are key cytokines involved in alerting the immune system to viral infection. After IFN stimulation, cellular transcriptional profile critically changes, leading to the expression of several IFN stimulated genes (ISGs) that exert a wide variety of antiviral activities. Despite many ISGs have been already identified, a comprehensive network of coding and non-coding genes with a central role in IFN-response still needs to be elucidated. We performed a global RNA-Seq transcriptome profile of the HCV permissive human hepatoma cell line Huh7.5 and its parental cell line Huh7, upon IFN treatment, to define a network of genes whose coordinated modulation plays a central role in IFN-response. Our study adds molecular actors, coding and non-coding genes, to the complex molecular network underlying IFN-response and shows how systems biology approaches, such as correlation networks, network's topology and gene ontology analyses can be leveraged to this aim.

Novel interferon-sensitive genes unveiled by correlation-driven gene selection and systems biology / C. Cheroni, L. Manganaro, L. Donnici, V. Bevilacqua, R.J.P. Bonnal, R.L. Rossi, R. De Francesco. - In: SCIENTIFIC REPORTS. - ISSN 2045-2322. - 11:1(2021 Sep 10), pp. 18043.1-18043.4. [10.1038/s41598-021-97258-8]

Novel interferon-sensitive genes unveiled by correlation-driven gene selection and systems biology

Cheroni, Cristina;Manganaro, Lara;Donnici, Lorena;Bevilacqua, Valeria;De Francesco, Raffaele
2021-09-10

Abstract

Interferons (IFNs) are key cytokines involved in alerting the immune system to viral infection. After IFN stimulation, cellular transcriptional profile critically changes, leading to the expression of several IFN stimulated genes (ISGs) that exert a wide variety of antiviral activities. Despite many ISGs have been already identified, a comprehensive network of coding and non-coding genes with a central role in IFN-response still needs to be elucidated. We performed a global RNA-Seq transcriptome profile of the HCV permissive human hepatoma cell line Huh7.5 and its parental cell line Huh7, upon IFN treatment, to define a network of genes whose coordinated modulation plays a central role in IFN-response. Our study adds molecular actors, coding and non-coding genes, to the complex molecular network underlying IFN-response and shows how systems biology approaches, such as correlation networks, network's topology and gene ontology analyses can be leveraged to this aim.
interferon; hepatitis C virus; HCV; interferon-sensitive genes
Settore BIO/19 - Microbiologia Generale
SCIENTIFIC REPORTS
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/867558
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