The pathogenesis of amyotrophic lateral sclerosis (ALS), a lethal neurodegenerative dis-ease, remains undisclosed. Mutations in ALS related genes have been identified, albeit the majority of cases are unmutated. Clinical pathology of ALS suggests a prion‐like cell‐to‐cell diffusion of the disease possibly mediated by exosomes, small endocytic vesicles involved in the propagation of RNA molecules and proteins. In this pilot study, we focused on exosomal microRNAs (miRNAs), key regulators of many signaling pathways. We analyzed serum‐derived exosomes from ALS patients in comparison with healthy donors. Exosomes were obtained by a commercial kit. Purifica-tion of miRNAs was performed using spin column chromatography and RNA was reverse tran-scribed into cDNA. All samples were run on the miRCURY LNA™ Universal RT miRNA PCR Serum/Plasma Focus panel. An average of 29 miRNAs were detectable per sample. The supervised analysis did not identify any statistically significant difference among the groups indicating that none of the miRNA of our panel has a strong pathological role in ALS. However, selecting samples with the highest miRNA content, six biological processes shared across miRNAs through the in-tersection of the GO categories were identified. Our results, combined to those reported in the lit-erature, indicated that further investigation is needed to elucidate the role of exosome‐derived miRNA in ALS.

Exosome micrornas in amyotrophic lateral sclerosis : A pilot study / F. Pregnolato, L. Cova, A. Doretti, D. Bardelli, V. Silani, P. Bossolasco. - In: BIOMOLECULES. - ISSN 2218-273X. - 11:8(2021), pp. 1220.1-1220.12. [10.3390/biom11081220]

Exosome micrornas in amyotrophic lateral sclerosis : A pilot study

V. Silani;
2021

Abstract

The pathogenesis of amyotrophic lateral sclerosis (ALS), a lethal neurodegenerative dis-ease, remains undisclosed. Mutations in ALS related genes have been identified, albeit the majority of cases are unmutated. Clinical pathology of ALS suggests a prion‐like cell‐to‐cell diffusion of the disease possibly mediated by exosomes, small endocytic vesicles involved in the propagation of RNA molecules and proteins. In this pilot study, we focused on exosomal microRNAs (miRNAs), key regulators of many signaling pathways. We analyzed serum‐derived exosomes from ALS patients in comparison with healthy donors. Exosomes were obtained by a commercial kit. Purifica-tion of miRNAs was performed using spin column chromatography and RNA was reverse tran-scribed into cDNA. All samples were run on the miRCURY LNA™ Universal RT miRNA PCR Serum/Plasma Focus panel. An average of 29 miRNAs were detectable per sample. The supervised analysis did not identify any statistically significant difference among the groups indicating that none of the miRNA of our panel has a strong pathological role in ALS. However, selecting samples with the highest miRNA content, six biological processes shared across miRNAs through the in-tersection of the GO categories were identified. Our results, combined to those reported in the lit-erature, indicated that further investigation is needed to elucidate the role of exosome‐derived miRNA in ALS.
Amyotrophic lateral sclerosis; Biomarkers; Exosome; MiRNA; Serum
Settore MED/26 - Neurologia
Settore BIO/12 - Biochimica Clinica e Biologia Molecolare Clinica
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/867468
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