Malaria remains the most important mosquito-borne infectious disease worldwide, with 229 million new cases and 409.000 deaths in 2019. The infection is caused by a protozoan parasite which attacks red blood cells by feeding on hemoglobin and transforming it into hemozoin. Despite the WHO recommendation of prompt malaria diagnosis, the quality of microscopy-based diagnosis is frequently inadequate while rapid diagnostic tests based on antigens are not quantitative and still affected by non-negligible false negative/positive results. PCR-based methods are highly performant but still not widely used in endemic areas. Here, a diagnostic tool (TMek), based on the paramagnetic properties of hemozoin nanocrystals in infected red blood cells (i-RBCs), is reported on. Exploiting the competition between gravity and magnetic forces, i-RBCs in a whole blood specimen are sorted and electrically detected in a microchip. The amplitude and time evolution of the electrical signal allow for the quantification of i-RBCs (in the range 10–105 i-RBC µL−1) and the distinction of the infection stage. A preliminary validation study on 75 patients with clinical suspect of malaria shows on-field operability, without false negative and a few false positive results. These findings indicate the potential of TMek as a quantitative, stage-selective, rapid test for malaria.

A Lab-On-chip Tool for Rapid, Quantitative, and Stage-selective Diagnosis of Malaria / M. Giacometti, F. Milesi, P.L. Coppadoro, A. Rizzo, F. Fagiani, C. Rinaldi, M. Cantoni, D. Petti, E. Albisetti, M. Sampietro, M. Ciardo, G. Siciliano, P. Alano, B. Lemen, J. Bombe, M.T. Nwaha Toukam, P.F. Tina, M.R. Gismondo, M. Corbellino, R. Grande, G.B. Fiore, G. Ferrari, S. Antinori, R. Bertacco. - In: ADVANCED SCIENCE. - ISSN 2198-3844. - 8:14(2021 Jul 21), pp. 2004101.1-2004101.12. [10.1002/advs.202004101]

A Lab-On-chip Tool for Rapid, Quantitative, and Stage-selective Diagnosis of Malaria

A. Rizzo;M.R. Gismondo;R. Grande;S. Antinori
Penultimo
Writing – Review & Editing
;
2021-07-21

Abstract

Malaria remains the most important mosquito-borne infectious disease worldwide, with 229 million new cases and 409.000 deaths in 2019. The infection is caused by a protozoan parasite which attacks red blood cells by feeding on hemoglobin and transforming it into hemozoin. Despite the WHO recommendation of prompt malaria diagnosis, the quality of microscopy-based diagnosis is frequently inadequate while rapid diagnostic tests based on antigens are not quantitative and still affected by non-negligible false negative/positive results. PCR-based methods are highly performant but still not widely used in endemic areas. Here, a diagnostic tool (TMek), based on the paramagnetic properties of hemozoin nanocrystals in infected red blood cells (i-RBCs), is reported on. Exploiting the competition between gravity and magnetic forces, i-RBCs in a whole blood specimen are sorted and electrically detected in a microchip. The amplitude and time evolution of the electrical signal allow for the quantification of i-RBCs (in the range 10–105 i-RBC µL−1) and the distinction of the infection stage. A preliminary validation study on 75 patients with clinical suspect of malaria shows on-field operability, without false negative and a few false positive results. These findings indicate the potential of TMek as a quantitative, stage-selective, rapid test for malaria.
diagnostic tests; hemozoin nanocrystals; impedimetric detection; lab-on-chip; magnetic sorting; malaria; red blood cells
Settore MED/17 - Malattie Infettive
Settore MED/07 - Microbiologia e Microbiologia Clinica
13-mag-2021
Article (author)
File in questo prodotto:
File Dimensione Formato  
advs.202004101.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 1.53 MB
Formato Adobe PDF
1.53 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/867424
Citazioni
  • ???jsp.display-item.citation.pmc??? 1
  • Scopus 2
  • ???jsp.display-item.citation.isi??? 2
social impact