Mode of action of farnesene, a volatile sesquiterpene commonly found in the essential oils of several plants, was deeply studied on the model species Arabidopsis thaliana. The effects of farnesene on the Arabidopsis root morphology were evaluated by different microscopic techniques. As well, microtubules immunolabeling, phytohormone measurements and ROS staining helped us to elucidate the single or multi-modes of action of this sesquiterpene on plant metabolism. Farnesene-treated roots showed a strong growth inhibition and marked modifications on morphology, important tissue alterations, cellular damages and anisotropic growth. Left-handed growth of farnesene-treated roots, reverted by taxol (a known microtubule stabilizer), was related to microtubule condensation and disorganization. As well, the inhibition of primary root growth, lateral root number, lateral root length, and both root hairs length and density could be explained by the strong increment in ethylene production and auxin content detected in farnesene-treated seedlings. Microtubule alteration and hormonal unbalance appear as important components in the mode of action of farnesene and confirm the strong phytotoxic potential of this sesquiterpene.

Loss of gravitropism in farnesene-treated arabidopsis is due to microtubule malformations related to hormonal and ROS unbalance / F. Araniti, E. Grana, U. Krasuska, R. Bogatek, M.J. Reigosa, M.R. Abenavoli, A.M. Sanchez-Moreiras. - In: PLOS ONE. - ISSN 1932-6203. - 11:8(2016), pp. e0160568.1-e0160568.26. [10.1371/journal.pone.0160202]

Loss of gravitropism in farnesene-treated arabidopsis is due to microtubule malformations related to hormonal and ROS unbalance

F. Araniti
;
2016

Abstract

Mode of action of farnesene, a volatile sesquiterpene commonly found in the essential oils of several plants, was deeply studied on the model species Arabidopsis thaliana. The effects of farnesene on the Arabidopsis root morphology were evaluated by different microscopic techniques. As well, microtubules immunolabeling, phytohormone measurements and ROS staining helped us to elucidate the single or multi-modes of action of this sesquiterpene on plant metabolism. Farnesene-treated roots showed a strong growth inhibition and marked modifications on morphology, important tissue alterations, cellular damages and anisotropic growth. Left-handed growth of farnesene-treated roots, reverted by taxol (a known microtubule stabilizer), was related to microtubule condensation and disorganization. As well, the inhibition of primary root growth, lateral root number, lateral root length, and both root hairs length and density could be explained by the strong increment in ethylene production and auxin content detected in farnesene-treated seedlings. Microtubule alteration and hormonal unbalance appear as important components in the mode of action of farnesene and confirm the strong phytotoxic potential of this sesquiterpene.
Arabidopsis; Ethylenes; Hydrogen Peroxide; Indoleacetic Acids; Microscopy, Electron, Transmission; Microtubules; Nitric Oxide; Paclitaxel; Plant Growth Regulators; Plant Roots; Reactive Oxygen Species; Seedlings; Sesquiterpenes; Superoxides; Gravitropism
Settore AGR/13 - Chimica Agraria
Article (author)
File in questo prodotto:
File Dimensione Formato  
2016-Loss of gravitropism in farnesene-treated arabidopsis is due to microtubule malformations related to hormonal and ROS unbalance_compressed.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 9.14 MB
Formato Adobe PDF
9.14 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/865222
Citazioni
  • ???jsp.display-item.citation.pmc??? 13
  • Scopus 37
  • ???jsp.display-item.citation.isi??? 30
social impact