Nitrogen (N) supply usually limits crop production and optimizing N-use efficiency (NUE) to minimize fertilizer loss is important. NUE is a complex trait that can be dissected into crop N uptake from the soil (NUpE) and N utilization (NUtE). We compared NUE in 14 genotypes of three week old tomatoes grown in sand or hydroponic culture supplied with nitrate (NO3-). Culture method influenced measured NUE for some cultivars, but Regina Ostuni (RO) and UC82 were consistently identified as high and low NUE genotypes. To identify why these genotypes had contrasting NUE some traits were compared growing under 0.1 and 5 mM NO3- supply. UC82 showed greater root 15NO3- influx at low and high supply, and stronger SlNRT2.1/NAR2.1 transporter expression under low supply when compared with RO. Conversely, RO showed a higher total root length and thickness compared to UC82. Compared with UC82, RO showed higher shoot SlNRT2.3 expression and NO3- storage at high supply, but similar NO3- reductase activity. After N-starvation, root cell electrical potentials of RO were significantly more negative than UC82, but nitrate elicited similar responses in both root types. Overall for UC82 and RO, NUtE may play a greater role than NUpE for improved NUE.

Phenotyping two tomato genotypes with different nitrogen use efficiency / M.R. Abenavoli, C. Longo, A. Lupini, A.J. Miller, F. Araniti, F. Mercati, M.P. Princi, F. Sunseri. - In: PLANT PHYSIOLOGY AND BIOCHEMISTRY. - ISSN 0981-9428. - 107(2016), pp. 21-32. [10.1016/j.plaphy.2016.04.021]

Phenotyping two tomato genotypes with different nitrogen use efficiency

F. Araniti;
2016

Abstract

Nitrogen (N) supply usually limits crop production and optimizing N-use efficiency (NUE) to minimize fertilizer loss is important. NUE is a complex trait that can be dissected into crop N uptake from the soil (NUpE) and N utilization (NUtE). We compared NUE in 14 genotypes of three week old tomatoes grown in sand or hydroponic culture supplied with nitrate (NO3-). Culture method influenced measured NUE for some cultivars, but Regina Ostuni (RO) and UC82 were consistently identified as high and low NUE genotypes. To identify why these genotypes had contrasting NUE some traits were compared growing under 0.1 and 5 mM NO3- supply. UC82 showed greater root 15NO3- influx at low and high supply, and stronger SlNRT2.1/NAR2.1 transporter expression under low supply when compared with RO. Conversely, RO showed a higher total root length and thickness compared to UC82. Compared with UC82, RO showed higher shoot SlNRT2.3 expression and NO3- storage at high supply, but similar NO3- reductase activity. After N-starvation, root cell electrical potentials of RO were significantly more negative than UC82, but nitrate elicited similar responses in both root types. Overall for UC82 and RO, NUtE may play a greater role than NUpE for improved NUE.
Nitrate influx; Nitrate reductase; Nitrate transporter genes; Nitrate uptake; Nitrogen Uptake Efficiency - NUpE; Nitrogen Utilization Efficiency - NUtE; Tomato (Solanum lycopersicon L.); Enzyme Assays; Gene Expression Regulation, Plant; Genotype; Hydroponics; Kinetics; Lycopersicon esculentum; Membrane Potentials; Nitrate Reductase; Nitrates; Nitrogen; Nitrogen Isotopes; Phenotype; Plant Roots; Plant Shoots
Settore AGR/13 - Chimica Agraria
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/865218
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