The ability to control neural activity is essential for research in basic neuroscience, as it affects the mental state of an individual, but also in clinical neurology for brain therapies or stimulation. Available forms of spatiotemporally controlled neuromodulation include transcranial-magnetic or current (AC/DC) stimulation as well as light-mediated control. Among the latter, optogenetics has revolutionized neuroscience, yet its clinical translation is hampered by the need of gene therapy. As a drug-based alternative, the development of a new photoswitchable muscarinic agonist (PAI) and its effect on a brain network is evaluated in this work. First, the conditions to manipulate muscarinic receptors with light in the experimental setup are determined. Then, the emergent cortical activity consisting on synchronous slow oscillations –as in slow wave sleep– is transformed into a higher frequency pattern in the cerebral cortex both in vitro and in vivo. These results open the way to study cholinergic neuromodulation and to control spatiotemporal patterns of activity in different brain states, their transitions, and their links to cognition and behavior. The approach can be applied to different organisms and does not require genetic manipulation, which makes it translational to humans.

Control of brain state transitions with a photoswitchable muscarinic agonist / A. Barbero-Castillo, F. Riefolo, C. Matera, S. Caldas-Martínez, P. Mateos-Aparicio, J.F. Weinert, E. Claro, R. Sortino, A. Garrido-Charles, M.V. Sanchez-Vives, P. Gorostiza - In: Abstracts of papers presented at the EMBO Workshop: Chemical Biology 2020[s.l] : EMBO, 2020. - pp. 38-38 (( convegno EMBO Workshop: Chemical Biology 2020 tenutosi a Virtual Workshop nel 2020.

Control of brain state transitions with a photoswitchable muscarinic agonist

F. Riefolo;C. Matera;
2020

Abstract

The ability to control neural activity is essential for research in basic neuroscience, as it affects the mental state of an individual, but also in clinical neurology for brain therapies or stimulation. Available forms of spatiotemporally controlled neuromodulation include transcranial-magnetic or current (AC/DC) stimulation as well as light-mediated control. Among the latter, optogenetics has revolutionized neuroscience, yet its clinical translation is hampered by the need of gene therapy. As a drug-based alternative, the development of a new photoswitchable muscarinic agonist (PAI) and its effect on a brain network is evaluated in this work. First, the conditions to manipulate muscarinic receptors with light in the experimental setup are determined. Then, the emergent cortical activity consisting on synchronous slow oscillations –as in slow wave sleep– is transformed into a higher frequency pattern in the cerebral cortex both in vitro and in vivo. These results open the way to study cholinergic neuromodulation and to control spatiotemporal patterns of activity in different brain states, their transitions, and their links to cognition and behavior. The approach can be applied to different organisms and does not require genetic manipulation, which makes it translational to humans.
Settore CHIM/08 - Chimica Farmaceutica
Settore BIO/14 - Farmacologia
https://www.embl.de/training/events/2020/CHB20-01/index.html
Book Part (author)
File in questo prodotto:
File Dimensione Formato  
CHB20-01_EMBL Abstract Book_19 Aug v5_unlckd.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 2.17 MB
Formato Adobe PDF
2.17 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/861388
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact