The use of lake sedimentary DNA to track the long-term changes in both terrestrial and aquatic biota is a rapidly advancing field in paleoecological research. Although largely applied nowadays, knowledge gaps remain in this field and there is therefore still research to be conducted to ensure the reliability of the sedimentary DNA signal. Building on the most recent literature and seven original case studies, we synthesize the state-of-the-art analytical procedures for effective sampling, extraction, amplification, quantification and/or generation of DNA inventories from sedimentary ancient DNA (sedaDNA) via high-throughput sequencing technologies. We provide recommendations based on current knowledge and best practises.

Lake sedimentary dna research on past terrestrial and aquatic biodiversity : Overview and recommendations / E. Capo, C. Giguet-Covex, A. Rouillard, K. Nota, P.D. Heintzman, A. Vuillemin, D. Ariztegui, F. Arnaud, S. Belle, S. Bertilsson, C. Bigler, R. Bindler, A.G. Brown, C.L. Clarke, S.E. Crump, D. Debroas, G. Englund, G.F. Ficetola, R.E. Garner, J. Gauthier, I. Gregory-Eaves, L. Heinecke, U. Herzschuh, A. Ibrahim, V. Kisand, K.H. Kjaer, Y. Lammers, J. Littlefair, E. Messager, M.-. Monchamp, F. Olajos, W. Orsi, M.W. Pedersen, D.P. Rijal, J. Rydberg, T. Spanbauer, K.R. Stoof-Leichsenring, P. Taberlet, L. Talas, C. Thomas, D.A. Walsh, Y. Wang, E. Willerslev, A. van Woerkom, H.H. Zimmermann, M.J.L. Coolen, L.S. Epp, I. Domaizon, I.G. Alsos, L. Parducci. - In: QUATERNARY. - ISSN 2571-550X. - 4:1(2021), pp. 6.1-6.58. [10.3390/quat4010006]

Lake sedimentary dna research on past terrestrial and aquatic biodiversity : Overview and recommendations

G.F. Ficetola;
2021

Abstract

The use of lake sedimentary DNA to track the long-term changes in both terrestrial and aquatic biota is a rapidly advancing field in paleoecological research. Although largely applied nowadays, knowledge gaps remain in this field and there is therefore still research to be conducted to ensure the reliability of the sedimentary DNA signal. Building on the most recent literature and seven original case studies, we synthesize the state-of-the-art analytical procedures for effective sampling, extraction, amplification, quantification and/or generation of DNA inventories from sedimentary ancient DNA (sedaDNA) via high-throughput sequencing technologies. We provide recommendations based on current knowledge and best practises.
Biodiversity; Lake sediments; Metabarcoding; Metagenomics; Paleoecology; Paleogenetics; Paleogenomics; Paleolimnology; Sedimentary ancient DNA; Sedimentary DNA
Settore BIO/05 - Zoologia
Settore BIO/03 - Botanica Ambientale e Applicata
Settore BIO/07 - Ecologia
Settore BIO/18 - Genetica
Settore GEO/01 - Paleontologia e Paleoecologia
Article (author)
File in questo prodotto:
File Dimensione Formato  
capo 2021 sedDNA.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 6.05 MB
Formato Adobe PDF
6.05 MB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/857245
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 58
  • ???jsp.display-item.citation.isi??? 47
social impact