Omics approaches have significantly impacted knowledge about molecular signaling pathways driving cell function. Induced pluripotent stem cells (iPSC) have revolutionized the field of biological sciences and proteomics and, in particular, has been instrumental in identifying key elements operating during the maintenance of the pluripotent state and the differentiation process to the diverse cell types that form organisms. This review covers the evolution of conceptual and methodological strategies in proteomics; briefly describes the generation of iPSC from a historical perspective, the state-of-the-art of iPSC-based proteomics; and compares data on the proteome and transcriptome of iPSC to that of embryonic stem cells (ESC). Finally, proteomics of healthy and diseased cells and organoids differentiated from iPSC are analyzed.

Proteomics in the World of Induced Pluripotent Stem Cells / R. Soares Lindoso, T.H. Kasai-Brunswick, G. Monnerat Cahli, F. Collino, A. Bastos Carvalho, A. Carlos Campos de Carvalho, A. Vieyra. - In: CELLS. - ISSN 2073-4409. - 8:7(2019), pp. 703.1-703.25. [10.3390/cells8070703]

Proteomics in the World of Induced Pluripotent Stem Cells

Federica Collino;
2019

Abstract

Omics approaches have significantly impacted knowledge about molecular signaling pathways driving cell function. Induced pluripotent stem cells (iPSC) have revolutionized the field of biological sciences and proteomics and, in particular, has been instrumental in identifying key elements operating during the maintenance of the pluripotent state and the differentiation process to the diverse cell types that form organisms. This review covers the evolution of conceptual and methodological strategies in proteomics; briefly describes the generation of iPSC from a historical perspective, the state-of-the-art of iPSC-based proteomics; and compares data on the proteome and transcriptome of iPSC to that of embryonic stem cells (ESC). Finally, proteomics of healthy and diseased cells and organoids differentiated from iPSC are analyzed.
differentiation; embryonic stem cells; induced pluripotent stem cells; organoids; proteomics; Cell Differentiation; Embryonic Stem Cells; Humans; Proteome; Proteomics; Induced Pluripotent Stem Cells; Transcriptome
Settore MED/46 - Scienze Tecniche di Medicina di Laboratorio
Settore BIO/13 - Biologia Applicata
CELLS
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/856808
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