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IRIS è il sistema di gestione integrata dei dati della ricerca (persone, progetti, pubblicazioni, attività) adottato dall'Università degli Studi di Milano.
AIR nasce in UNIMI nel 2006 con lo scopo di raccogliere, documentare e conservare le informazioni sulla produzione scientifica dell'Università degli Studi di Milano e costituisce di fatto l'Anagrafe della ricerca dell'Ateneo.
EnglishNewly generated neurons pass through a series of well-defined developmental stages, which allow them to integrate into existing neuronal circuits. After exit from the cell cycle, postmitotic neurons undergo neuronal migration, axonal elongation, axon pruning, dendrite morphogenesis and synaptic maturation and plasticity. Lack of a global metabolic analysis during early cortical neuronal development led us to explore the role of cellular metabolism and mitochondrial biology during ex vivo differentiation of primary cortical neurons. Unexpectedly, we observed a huge increase in mitochondrial biogenesis. Changes in mitochondrial mass, morphology and function were correlated with the upregulation of the master regulators of mitochondrial biogenesis, TFAM and PGC-1α. Concomitant with mitochondrial biogenesis, we observed an increase in glucose metabolism during neuronal differentiation, which was linked to an increase in glucose uptake and enhanced GLUT3 mRNA expression and platelet isoform of phosphofructokinase 1 (PFKp) protein expression. In addition, glutamate-glutamine metabolism was also increased during the differentiation of cortical neurons. We identified PI3K-Akt-mTOR signalling as a critical regulator role of energy metabolism in neurons. Selective pharmacological inhibition of these metabolic pathways indicate existence of metabolic checkpoint that need to be satisfied in order to allow neuronal differentiation.
Metabolic reprogramming during neuronal differentiation / M. Agostini, F. Romeo, S. Inoue, M.V. Niklison-Chirou, A.J. Elia, D. Dinsdale, N. Morone, R.A. Knight, T.W. Mak, G. Melino. - In: CELL DEATH AND DIFFERENTIATION. - ISSN 1350-9047. - 23:9(2016), pp. 1502-1514. [10.1038/cdd.2016.36]
| Titolo: | Metabolic reprogramming during neuronal differentiation | |
| Autori: | ROMEO, FRANCESCO (Co-primo) | |
| Parole Chiave: | Animals; DNA, Mitochondrial; Glucose; Glucose Transporter Type 3; Glutamate-Cysteine Ligase; Glutamic Acid; Glutamine; Mice; Mice, Knockout; Microscopy, Electron, Transmission; Mitochondria; Neurons; Peroxisome Proliferator-Activated Receptor Gamma Coactivator 1-alpha; Phosphatidylinositol 3-Kinases; Proto-Oncogene Proteins c-akt; Reactive Oxygen Species; Signal Transduction; TOR Serine-Threonine Kinases; Cell Differentiation; Metabolic Engineering | |
| Settore Scientifico Disciplinare: | Settore BIO/10 - Biochimica Settore BIO/11 - Biologia Molecolare Settore MED/04 - Patologia Generale | |
| Data di pubblicazione: | 2016 | |
| Rivista: | ||
| Tipologia: | Article (author) | |
| Digital Object Identifier (DOI): | http://dx.doi.org/10.1038/cdd.2016.36 | |
| Appare nelle tipologie: | 01 - Articolo su periodico |
File in questo prodotto:
| File | Descrizione | Tipologia | Licenza | |
|---|---|---|---|---|
| cdd201636.pdf | Publisher's version/PDF | Open Access Visualizza/Apri |
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