Laser and neutron scattering experiments showed that in mixed micelles of ganglioside GM2 and GT1b, a membrane mimicking system, the segregation of gangliosides may occur spontaneously. Photolabeling experiments using nitrophenylazide containing ganglioside GM1 proved that gangliosides added to cells in culture enter the cell and bind to its membrane as components of microdomains, which specifically interact with a protein of about 30 kDa. This suggest that ganglioside segregation may be a natural phenomenon. Gangliosides when added to granule cells in culture led to increase in protein phosphorylation, the effect exerted being related to the amount of ganglioside molecules inserted stably into the cell lipid layer and an increase of 0.7% of the cell original ganglioside content promoted an increase of 57% in the incorporation of P-32 into cell membrane proteins. From the above results a possible relationship between ganglioside segregation and involvement of ganglioside in enzyme activity control is suggested.

Relationship between the regulation of membrane enzyme-activities y gangliosides and a possible ganglioside segregation in membrane microdomains / S. Sonnino, V. Chigorno, M. Valsecchi, R. Bassi, D. Acquotti, L. Cantu, M. Corti, G. Tettamanti. - In: INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS. - ISSN 0301-1208. - 27:6(1990), pp. 353-358. ((Intervento presentato al 2. convegno International symp on biochemical role of eukaryotic cell surface macromolecules tenutosi a Delhi nel 1990.

Relationship between the regulation of membrane enzyme-activities y gangliosides and a possible ganglioside segregation in membrane microdomains

S. SONNINO;V. CHIGORNO;M. VALSECCHI;R. BASSI;L. CANTU;M. CORTI;G. TETTAMANTI
1990

Abstract

Laser and neutron scattering experiments showed that in mixed micelles of ganglioside GM2 and GT1b, a membrane mimicking system, the segregation of gangliosides may occur spontaneously. Photolabeling experiments using nitrophenylazide containing ganglioside GM1 proved that gangliosides added to cells in culture enter the cell and bind to its membrane as components of microdomains, which specifically interact with a protein of about 30 kDa. This suggest that ganglioside segregation may be a natural phenomenon. Gangliosides when added to granule cells in culture led to increase in protein phosphorylation, the effect exerted being related to the amount of ganglioside molecules inserted stably into the cell lipid layer and an increase of 0.7% of the cell original ganglioside content promoted an increase of 57% in the incorporation of P-32 into cell membrane proteins. From the above results a possible relationship between ganglioside segregation and involvement of ganglioside in enzyme activity control is suggested.
Settore BIO/10 - Biochimica
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
INDIAN JOURNAL OF BIOCHEMISTRY & BIOPHYSICS
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/853639
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