C-peptide therapy protects against diabetic micro- and macrovascular damages and neuropatic complications. However, to date, the role of C-peptide in preventing diabetes-related bone loss has not been investigated. Our aim was to evaluate if C-peptide infusion improves bone quality in diabetic rats. Twenty-three male Wistar rats were randomly divided into three groups: normal control group; sham diabetic control group; diabetic plus C-peptide group. Diabetes was induced by streptozotocin injection and C-peptide was delivered subcutaneously for 6 weeks. We performed micro-CT and histological testing to assess several trabecular microarchitectural parameters. At the end, diabetic plus C-peptide rats had a higher serum C-peptide (p = 0.02) and calcium (p = 0.04) levels and tibia weight (p = 0.02) than the diabetic control group. The diabetic plus C-peptide group showed a higher trabecular thickness and cross-sectional thickness than the diabetic control group (p = 0.01 and p = 0.03). Both the normal control and diabetic plus C-peptide groups had more Runx-2 and PLIN1 positive cells in comparison with the diabetic control group (p = 0.045 and p = 0.034). Diabetic rats receiving C-peptide had higher quality of trabecular bone than diabetic rats not receiving this treatment. If confirmed, C-peptide could have a role in improving bone quality in diabetes.
Effects of C-Peptide Replacement Therapy on Bone Microarchitecture Parameters in Streptozotocin-Diabetic Rats / S. Maurotti, C. Russo, V. Musolino, S. Nucera, M. Gliozzi, M. Scicchitano, F. Bosco, V.M. Morittu, M. Ragusa, E. Mazza, R. Pujia, C. Gazzaruso, D. Britti, M.T. Valenti, M. Deiana, S. Romeo, S. Giannini, L. Dalle Carbonare, V. Mollace, A. Pujia, T. Montalcini. - In: CALCIFIED TISSUE INTERNATIONAL. - ISSN 0171-967X. - 107:3(2020), pp. 266-280. [10.1007/s00223-020-00716-0]
|Titolo:||Effects of C-Peptide Replacement Therapy on Bone Microarchitecture Parameters in Streptozotocin-Diabetic Rats|
|Parole Chiave:||c-peptide; Bone microarchitectural parameters; Type 1 diabetes mellitus; Osteoporosis; Diabetic rat model|
|Settore Scientifico Disciplinare:||Settore MED/13 - Endocrinologia|
|Data di pubblicazione:||2020|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1007/s00223-020-00716-0|
|Appare nelle tipologie:||01 - Articolo su periodico|