In vitro cell transformation assays (CTAs) have been shown to model important stages of in vivo carcinogenesis and have the potential to predict carcinogenicity in humans. Advantages of CTAs are their ability of revealing both genotoxic and non-genotoxic carcinogens while reducing both experimental costs and the number of animals used. The endpoint of the CTA is foci formation, and requires classification under light microscopy based on morphology. Thus current limitations for the wide adoption of the assay partially depend on a fair degree of subjectivity in foci scoring. An objective evaluation may be obtained after separating foci from background monolayer in the digital image, and quantifying values of statistical descriptors which are selected to capture eye-scored morphological features. The aim of this study was to develop statistical descriptors to be applied to transformed foci of BALB/c 3T3, which cover foci size, multilayering and invasive cell growth into the background monolayer. Proposed descriptors were applied to a database of 407 foci images to explore the numerical features, and to illustrate open problems and potential solutions.
Objective scoring of transformed foci in BALB/c 3T3 cell transformation assay by statistical image descriptors / C. Urani, R. Corvi, G. Callegaro, F.M. Stefanini. - In: TOXICOLOGY IN VITRO. - ISSN 0887-2333. - 27:6(2013), pp. 1905-1912.
|Titolo:||Objective scoring of transformed foci in BALB/c 3T3 cell transformation assay by statistical image descriptors|
STEFANINI, FEDERICO MATTIA (Ultimo)
|Parole Chiave:||Statistical image descriptors; Morphological features; Cell transformation assay; BALB/c 3T3 cell line; Foci|
|Settore Scientifico Disciplinare:||Settore SECS-S/01 - Statistica|
|Data di pubblicazione:||2013|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1016/j.tiv.2013.06.011|
|Appare nelle tipologie:||01 - Articolo su periodico|
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