Background: Few allergic reactions to grape are reported in the literature. In some cases an association with peach and cherry allergy was observed. No IgE-mediated reactions to wine have been described, and no grape major allergens have yet been identified. Objective: We describe several severe reactions to grape or wine. We characterized the grape major allergens and tried to identify the allergen in wine. Methods: We collected documented histories of allergic reactions to grape and wine. Grape allergens were identified by means of SDS-PAGE and immunoblotting and purified by means of HPLC. Using amino acid sequencing and mass spectrometry, we identified the family of proteins to which the allergens belong. Cross-reactivity with peach and cherry was evaluated by means of cross-wise inhibition experiments. Results: Eleven patients with reactions to grape and 3 with anaphylactic reactions to wine were recruited. The major allergens were an endochitinase 4A and a lipid-transfer protein (LTP) that was homologous to and cross-reactive with peach LTP. A 24-kd protein homologous to the cherry thaumatin-like allergen was a minor allergen. Endochitinase 4A is very likely the allergen in vino novello and in vino Fragolino. Conclusions: Grape and wine might cause severe allergic reactions in sensitive patients. The major allergens of grape are endochitinase 4A, which is also the allergen of wine, and an LTP cross-reacting with the peach major allergen.
Identification of Grape and Wine Allergen as an Endochitinasis 4, a Lipid Transfer Protein and a Thaumatin / E.A. Pastorello, L. Farioli, V. Pravettoni, C. Ortolani, D. Fortunato, M.G. Giuffrida, L. Perono Garoffo, M.A. Calamari, O. Brenna, C. Conti. - In: JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY. - ISSN 0091-6749. - 111:2(2003), pp. 350-359.
|Titolo:||Identification of Grape and Wine Allergen as an Endochitinasis 4, a Lipid Transfer Protein and a Thaumatin|
PASTORELLO, ELIDE ANNA (Primo)
BRENNA, ORESTE VITTORE (Penultimo)
|Parole Chiave:||Food hypersensitivity; Grape; Major allergens; Minor allergens; Wine|
|Settore Scientifico Disciplinare:||Settore MED/09 - Medicina Interna|
Settore AGR/15 - Scienze e Tecnologie Alimentari
|Data di pubblicazione:||2003|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1067/mai.2003.35|
|Appare nelle tipologie:||01 - Articolo su periodico|