Tabby patterns of fur coats are defining characteristics in wild and domestic felids. Historically, three autosomal alleles at one locus (Tabby): Abyssinian (Ta ; a.k.a. ticked), mackerel (Tm ; a.k.a. striped) and blotched (tb ; a.k.a. classic, blotched) were thought to control these patterns in domestic cats and their breeds. Currently, at least three loci influence cat tabby markings, two of which are designated Tabby and Ticked. The Tabby locus is laeverin (LVRN) and affects the mackerel and blotched patterns. The unidentified gene for the Ticked locus on cat chromosome B1 was suggested to control the presence or absence of the ticked pattern (Tabby - Abyssinian (Ta ; a.k.a. ticked). The cat reference genome (Cinnamon, the Abyssinian) has the ticked phenotype and the variant dataset and coat phenotypes from the 99 Lives Cat Genome Consortium (195 cats) were used to identify candidate genes and variants associated with the Ticked locus. Two strategies were used to find the Ticked allele(s), one considered Cinnamon with the reference allele or heterozygous (Strategy A) and the other considered Cinnamon as having the variant allele or heterozygous (Strategy B). For Strategy A, two variants in Dickkopf Wnt Signaling Pathway Inhibitor 4 (DKK4), a p.Cys63Tyr (B1:41621481, c.188G>A) and a less common p.Ala18Val (B1:42620835, c.53C>T) variant are suggested as two alleles influencing the Ticked phenotype. Bioinformatic and molecular modeling analysis suggests that these changes disrupt a key disulfide bond in the Dkk4 cysteine-rich domain 1 or Dkk4 signal peptide cleavage respectively. All coding variants were excluded as Ticked alleles using Strategy B.

Mining the 99 Lives Cat Genome Sequencing Consortium database implicates genes and variants for the Ticked locus in domestic cats (Felis catus) / L. A Lyons, R. M Buckley, R. J Harvey, 9. Lives Cat Genome Consortium: Marie Abitbol, D. Aberdein, P. C Alves, A. Ohlsson Andersson, R. R Bellone, T. F Bergström, N. Bilgen, A. R Boyko, J. A Brockman, M. L Casal, M. G Castelhano, B. W Davis, L. Davison, O. Distl, N. H Dodman, N. Matthew Ellinwood, J. E Fogle, O. P Forman, D. J Garrick, E. I Ginns, J. Häggström, D. Hasegawa, B. Haase, V. Jagannathan, P. Lait, I. Hernandez, M. K Hytönen, M. Kaukonen, T. Kosho, E. Leclerc, T. L Lear, T. Leeb, R.H. L Li, H. Lohi, M.L. Longeri, M. A Magnuson, R. Malik, S. P Mane, R. Middleton, J. S Munday, W. J Murphy, A. N Myers, N. C Pedersen, S. M Peterson-Jones, M. F Rothschild, C. Rusbridge, J. J Schoenebeck, B. Shapiro, J. A Stern, W. F Swanson, K. A Terio, R. J Todhunter, W. C Warren, E. A Wilcox, J. H Wildschutte, Y. Yu. - In: ANIMAL GENETICS. - ISSN 0268-9146. - 52:3(2021 Jun), pp. 321-332.

Mining the 99 Lives Cat Genome Sequencing Consortium database implicates genes and variants for the Ticked locus in domestic cats (Felis catus)

M.L. Longeri
Membro del Collaboration Group
;
2021

Abstract

Tabby patterns of fur coats are defining characteristics in wild and domestic felids. Historically, three autosomal alleles at one locus (Tabby): Abyssinian (Ta ; a.k.a. ticked), mackerel (Tm ; a.k.a. striped) and blotched (tb ; a.k.a. classic, blotched) were thought to control these patterns in domestic cats and their breeds. Currently, at least three loci influence cat tabby markings, two of which are designated Tabby and Ticked. The Tabby locus is laeverin (LVRN) and affects the mackerel and blotched patterns. The unidentified gene for the Ticked locus on cat chromosome B1 was suggested to control the presence or absence of the ticked pattern (Tabby - Abyssinian (Ta ; a.k.a. ticked). The cat reference genome (Cinnamon, the Abyssinian) has the ticked phenotype and the variant dataset and coat phenotypes from the 99 Lives Cat Genome Consortium (195 cats) were used to identify candidate genes and variants associated with the Ticked locus. Two strategies were used to find the Ticked allele(s), one considered Cinnamon with the reference allele or heterozygous (Strategy A) and the other considered Cinnamon as having the variant allele or heterozygous (Strategy B). For Strategy A, two variants in Dickkopf Wnt Signaling Pathway Inhibitor 4 (DKK4), a p.Cys63Tyr (B1:41621481, c.188G>A) and a less common p.Ala18Val (B1:42620835, c.53C>T) variant are suggested as two alleles influencing the Ticked phenotype. Bioinformatic and molecular modeling analysis suggests that these changes disrupt a key disulfide bond in the Dkk4 cysteine-rich domain 1 or Dkk4 signal peptide cleavage respectively. All coding variants were excluded as Ticked alleles using Strategy B.
DKK4; Dickkopf Wnt Signaling Pathway Inhibitor 4; Tabby; Abyssinian; coat pattern
Settore AGR/17 - Zootecnica Generale e Miglioramento Genetico
Article (author)
File in questo prodotto:
File Dimensione Formato  
2021 Animal Genetics Ticked.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 1.3 MB
Formato Adobe PDF
1.3 MB Adobe PDF Visualizza/Apri
age.13059.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 868.47 kB
Formato Adobe PDF
868.47 kB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/836796
Citazioni
  • ???jsp.display-item.citation.pmc??? 25
  • Scopus 85
  • ???jsp.display-item.citation.isi??? 4
social impact