Introduction: The World Health Organization (WHO) classification of canine nodal lymphomas is mainly based on histopathology and immunohistochemistry (HIS+IHC). The invasive sampling procedure and the reporting time represent a deterrent to the execution of this test, and many clinicians rely on cytopathology only. The aim of the present study was to assess the diagnostic performances of cytopathology in identifying: 1) canine nodal lymphoma, 2) immunophenotype and grade, 3) WHO histotype. Material & Methods: 161 cytopathological glass slides from enlarged peripheral lymph nodes were blindly submitted to 6 examiners. Final HIS+IHC diagnoses were obtained. The examiners indicated: 1) lymphoma vs non-lymphoma; 2) supposed grade and immunophenotype; 3) supposed WHO histotype. Results: Cytopathology was accurate and precise in identifying lymphoma samples (sensitivity = 93.1%, specificity = 89.5%, Kappa inter-observer agreement = 0.79). Agreement was only moderate in identifying grade and phenotype (k= 0.41) and WHO subtype (k=0.41). Only 48.6% evaluations agreed with final diagnosis according to grade and phenotype and 38.7% according to the WHO subtype. Conclusions: Cytopathology alone may accurately diagnose lymphoma but its accuracy drops when further characterization is needed. When B-cell lymphoma is suspected cytopathologically, the phenotype is likely to be confirmed via HIS+IHC. Caution should be made in reporting a high-grade lymphoma by cytopathology, particularly if B-cell phenotype is suspected. Histopathology and IHC are mandatory for a definitive diagnosis of the lymphoma WHO histotype. The role of further diagnostic techniques should be addressed in future studies.

Diagnostic performances of lymph node cytopathology in predicting lymphoma and its WHO histotype in dogs / V. Martini, L. Aresu, U. Bonfanti, M. Caniatti, F. Cian, M. Gambini, L. Marconato, C. Masserdotti, A. Nicoletti, F. Riondato, P. Roccabianca, D. Stefanello, E. Teske, S. Comazzi. ((Intervento presentato al convegno Virtual ESVONC Congress tenutosi a online nel 2021.

Diagnostic performances of lymph node cytopathology in predicting lymphoma and its WHO histotype in dogs

V. Martini
Primo
;
M. Caniatti;M. Gambini;P. Roccabianca;D. Stefanello;S. Comazzi
2021

Abstract

Introduction: The World Health Organization (WHO) classification of canine nodal lymphomas is mainly based on histopathology and immunohistochemistry (HIS+IHC). The invasive sampling procedure and the reporting time represent a deterrent to the execution of this test, and many clinicians rely on cytopathology only. The aim of the present study was to assess the diagnostic performances of cytopathology in identifying: 1) canine nodal lymphoma, 2) immunophenotype and grade, 3) WHO histotype. Material & Methods: 161 cytopathological glass slides from enlarged peripheral lymph nodes were blindly submitted to 6 examiners. Final HIS+IHC diagnoses were obtained. The examiners indicated: 1) lymphoma vs non-lymphoma; 2) supposed grade and immunophenotype; 3) supposed WHO histotype. Results: Cytopathology was accurate and precise in identifying lymphoma samples (sensitivity = 93.1%, specificity = 89.5%, Kappa inter-observer agreement = 0.79). Agreement was only moderate in identifying grade and phenotype (k= 0.41) and WHO subtype (k=0.41). Only 48.6% evaluations agreed with final diagnosis according to grade and phenotype and 38.7% according to the WHO subtype. Conclusions: Cytopathology alone may accurately diagnose lymphoma but its accuracy drops when further characterization is needed. When B-cell lymphoma is suspected cytopathologically, the phenotype is likely to be confirmed via HIS+IHC. Caution should be made in reporting a high-grade lymphoma by cytopathology, particularly if B-cell phenotype is suspected. Histopathology and IHC are mandatory for a definitive diagnosis of the lymphoma WHO histotype. The role of further diagnostic techniques should be addressed in future studies.
Settore VET/03 - Patologia Generale e Anatomia Patologica Veterinaria
Settore VET/08 - Clinica Medica Veterinaria
European Society of Veterinary Oncology
Diagnostic performances of lymph node cytopathology in predicting lymphoma and its WHO histotype in dogs / V. Martini, L. Aresu, U. Bonfanti, M. Caniatti, F. Cian, M. Gambini, L. Marconato, C. Masserdotti, A. Nicoletti, F. Riondato, P. Roccabianca, D. Stefanello, E. Teske, S. Comazzi. ((Intervento presentato al convegno Virtual ESVONC Congress tenutosi a online nel 2021.
Conference Object
File in questo prodotto:
File Dimensione Formato  
Abstract ESVONC Martini def.pdf

accesso riservato

Tipologia: Altro
Dimensione 104.31 kB
Formato Adobe PDF
104.31 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/832465
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact