Cats are susceptible to infection with severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2). Whilst a number of studies have been performed worldwide on owned cats, limited data are available on stray, colony or shelter cats. We investigated SARS-CoV-2 infection in a stray cat population before and during human outbreaks of SARS-CoV-2 in cities in the Lombardy region in northern Italy, a high endemic region for SARS-CoV-2, using serological and molecular methods. A cohort of different samples were collected from 241 cats, including frozen archived serum samples from 136 cats collected before the 2019 coronavirus disease (COVID-19) pandemic and serum, pharyngeal and rectal swab samples from 105 cats collected during the SARS-CoV-2 outbreak. All pre- pandemic samples tested seronegative for antibodies against the nucleocapsid of SARS-CoV-2 using indirect enzyme linked immunosorbent assay (ELISA) test, while one serum sample collected during the pandemic was seropositive. No serological cross-reactivity was detected between SARS-CoV-2 antibodies and antibodies against feline enteric (FECV) and infectious peritonitis coronavirus (FIPC), Feline Immunodeficiency Virus (FIV), Feline Calicivirus (FCV), Feline Herpesvirus-1 (FHV-1), Feline Parvovirus (FPV), Leishmania infantum, Anaplasma phagocytophilum, Rickettsia spp., Toxoplasma gondii or Chlamydophila felis. No pharyngeal or rectal swab tested positive for SARS-CoV-2 RNA on real time reverse transcription-polymerase chain reaction (rRT-PCR). Our data show that SARS-CoV-2 did infect stray cats in Lombardy during the COVID-19 pandemic, but with lower prevalence than found in owned cats. This should alleviate public concerns about stray cats acting as SARS-CoV-2 carriers.
A pre- and during Pandemic Survey of Sars-Cov-2 Infection in Stray Colony and Shelter Cats from a High Endemic Area of Northern Italy / E. Spada, F. Vitale, F. Bruno, G. Castelli, S. Reale, R. Perego, L. Baggiani, D. Proverbio. - In: VIRUSES. - ISSN 1999-4915. - 13:4(2021), pp. 618.1-618.11.
|Titolo:||A pre- and during Pandemic Survey of Sars-Cov-2 Infection in Stray Colony and Shelter Cats from a High Endemic Area of Northern Italy|
SPADA, EVA (Primo) [Conceptualization] (Corresponding)
PEREGO, ROBERTA [Writing – Review & Editing] (Corresponding)
BAGGIANI, LUCIANA (Penultimo) [Methodology]
PROVERBIO, DANIELA (Ultimo) [Conceptualization]
|Parole Chiave:||COVID-19; ELISA; RT-PCR; SARS-CoV-2; northern Italy; shelter cats; stray cat|
|Settore Scientifico Disciplinare:||Settore VET/08 - Clinica Medica Veterinaria|
Settore VET/05 - Malattie Infettive degli Animali Domestici
|Data di pubblicazione:||2021|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3390/v13040618|
|Appare nelle tipologie:||01 - Articolo su periodico|