In holometabolous insects, various larval organs are remodeled by autophagy during metamorphosis. Although moths and butterflies are among the first animal models in which this self-eating process was described, only in recent years autophagy has been analyzed in detail in these insects. In particular, the silkworm Bombyx mori, which represents a well-studied model among Lepidoptera, provides a wide repertoire of cellular and molecular tools useful for studying the occurrence of autophagy and for evaluating its role in postembryonic development. Here, we describe some morphological, biochemical, and molecular methods to monitor autophagy in silkworm organs.

Methods for monitoring autophagy in silkworm organs / A. Montali, M. Casartelli, D. Bruno, A. Grimaldi, G. Tettamanti (METHODS IN MOLECULAR BIOLOGY). - In: Autophagy in Differentiation and Tissue Maintenance / [a cura di] K. Turksen. - [s.l] : Humana Press Inc., 2019. - ISBN 9781493987474. - pp. 159-174

Methods for monitoring autophagy in silkworm organs

M. Casartelli;
2019

Abstract

In holometabolous insects, various larval organs are remodeled by autophagy during metamorphosis. Although moths and butterflies are among the first animal models in which this self-eating process was described, only in recent years autophagy has been analyzed in detail in these insects. In particular, the silkworm Bombyx mori, which represents a well-studied model among Lepidoptera, provides a wide repertoire of cellular and molecular tools useful for studying the occurrence of autophagy and for evaluating its role in postembryonic development. Here, we describe some morphological, biochemical, and molecular methods to monitor autophagy in silkworm organs.
Acid phosphatase; Atg8; Autophagy, Bombyx mori; Gene expression; Metamorphosis; Midgut; Silk gland; Transmission electron microscopy
Settore AGR/11 - Entomologia Generale e Applicata
Settore BIO/05 - Zoologia
Book Part (author)
File in questo prodotto:
File Dimensione Formato  
Montali2019_Protocol_MethodsForMonitoringAutophagyI.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 399.48 kB
Formato Adobe PDF
399.48 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/830198
Citazioni
  • ???jsp.display-item.citation.pmc??? 0
  • Scopus 2
  • ???jsp.display-item.citation.isi??? 0
social impact