We investigated how the shape of polymeric vesicles, made by the exact same material, impacts the replication activity and metabolic state of both cancer and non-cancer cell types. First, we isolated discrete geometrical structures (spheres and tubes) from a heterogeneous sample using density-gradient centrifugation. Then, we characterized the cellular internalization and the kinetics of uptake of both types of polymersomes in different cell types (either cancer or non-cancer cells). We also investigated the cellular metabolic response as a function of the shape of the structures internalized and discovered that tubular vesicles induce a significant decrease in the replication activity of cancer cells compared to spherical vesicles. We related this effect to the significant up-regulation of the tumor suppressor genes p21 and p53 with a concomitant activation of caspase 3/7. Finally, we demonstrated that combining the intrinsic shape-dependent effects of tubes with the delivery of doxorubicin significantly increases the cytotoxicity of the system. Our results illustrate how the geometrical conformation of nanoparticles could impact cell behavior and how this could be tuned to create novel drug delivery systems tailored to specific biomedical application.

Tuning cell behavior with nanoparticle shape / E. Scarpa, C. de Pace, A.S. Joseph, S.C. de Souza, A. Poma, E. Liatsi-Douvitsa, C. Contini, V. de Matteis, J.S. Marti, G. Battaglia, L. Rizzello. - In: PLOS ONE. - ISSN 1932-6203. - 15:11(2020), pp. e0240197.1-e0240197.16. [10.1371/journal.pone.0240197]

Tuning cell behavior with nanoparticle shape

E. Scarpa;A. Poma;L. Rizzello
Ultimo
2020

Abstract

We investigated how the shape of polymeric vesicles, made by the exact same material, impacts the replication activity and metabolic state of both cancer and non-cancer cell types. First, we isolated discrete geometrical structures (spheres and tubes) from a heterogeneous sample using density-gradient centrifugation. Then, we characterized the cellular internalization and the kinetics of uptake of both types of polymersomes in different cell types (either cancer or non-cancer cells). We also investigated the cellular metabolic response as a function of the shape of the structures internalized and discovered that tubular vesicles induce a significant decrease in the replication activity of cancer cells compared to spherical vesicles. We related this effect to the significant up-regulation of the tumor suppressor genes p21 and p53 with a concomitant activation of caspase 3/7. Finally, we demonstrated that combining the intrinsic shape-dependent effects of tubes with the delivery of doxorubicin significantly increases the cytotoxicity of the system. Our results illustrate how the geometrical conformation of nanoparticles could impact cell behavior and how this could be tuned to create novel drug delivery systems tailored to specific biomedical application.
Caspase 3; Caspase 7; Cell Line, Tumor; Centrifugation, Density Gradient; Cyclin-Dependent Kinase Inhibitor p21; DNA Replication; Doxorubicin; HeLa Cells; Humans; Nanoparticles; Neoplasms; Tumor Suppressor Protein p53; Up-Regulation
Settore BIO/10 - Biochimica
Settore CHIM/08 - Chimica Farmaceutica
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/827938
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