Sensitized triplet-triplet annihilation based photon up-conversion (TTA-UC) greatly improves the scope and applicability of fluorescence bioimaging by enabling anti-Stokes detection at low powers, thus eliminating the background autofluorescence and limiting the potential damage of the living tissues. Here the authors present a facile, one-step protocol to prepare dual dye-doped, TTA-UC active nanomicelles starting from the commercially available surfactant Kolliphor EL, a component of several FDA approved preparations. These nanosized micelles show an unprecedented up-conversion yield of 6.5% under 0.1 W cm(-2) excitation intensity in an aqueous, non-deaerated dispersion. The supramolecular architecture obtained preserves the embedded dyes from oxygen quenching, allowing satisfactory anti-Stokes fluorescence imaging of 3T3 cells. This is the first example of efficient multicomponent up-converters prepared using highly biocompatible materials approved by the international authority, paving the way for the development of new complex and multifunctional materials for advanced theranostics.

Self-Assembled Dual Dye-Doped Nanosized Micelles for High-Contrast Up-Conversion Bioimaging / S. Mattiello, A. Monguzzi, J. Pedrini, M. Sassi, C. Villa, Y. Torrente, R. Marotta, F. Meinardi, L. Beverina. - In: ADVANCED FUNCTIONAL MATERIALS. - ISSN 1616-3028. - 26:46(2016 Dec 13), pp. 8447-8454.

Self-Assembled Dual Dye-Doped Nanosized Micelles for High-Contrast Up-Conversion Bioimaging

C. Villa;Y. Torrente;
2016

Abstract

Sensitized triplet-triplet annihilation based photon up-conversion (TTA-UC) greatly improves the scope and applicability of fluorescence bioimaging by enabling anti-Stokes detection at low powers, thus eliminating the background autofluorescence and limiting the potential damage of the living tissues. Here the authors present a facile, one-step protocol to prepare dual dye-doped, TTA-UC active nanomicelles starting from the commercially available surfactant Kolliphor EL, a component of several FDA approved preparations. These nanosized micelles show an unprecedented up-conversion yield of 6.5% under 0.1 W cm(-2) excitation intensity in an aqueous, non-deaerated dispersion. The supramolecular architecture obtained preserves the embedded dyes from oxygen quenching, allowing satisfactory anti-Stokes fluorescence imaging of 3T3 cells. This is the first example of efficient multicomponent up-converters prepared using highly biocompatible materials approved by the international authority, paving the way for the development of new complex and multifunctional materials for advanced theranostics.
Settore MED/26 - Neurologia
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/826315
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