We investigated the effects of heliox administration (80% Helium in O2) on tidal inspiratory flow limitation (tIFL) occurring in supine anesthetized spontaneously breathing rabbits, regarded as an animal model of obstructive apnea-hypopnea syndrome. 22 rabbits were instrumented to record oro-nasal mask flow, airway opening, tracheal and esophageal pressures and diaphragm and genioglossus electromyographic activities while breathing either room air or heliox, and, in 12 rabbits, also during the application of continuous positive airway pressure (CPAP; 6 cmH2O). For the group, heliox increased peak inspiratory flow, ventilation (18±11%), peak inspiratory tracheal and dynamic transpulmonary pressures, but in no animal eliminated tIFL, as instead CPAP did in all. Muscle activities were unaffected by heliox. In the presence of IFL the increase in flow with heliox (ΔV̇IFL) varied markedly among rabbits (2 to 49%), allowing the distinction between responders and non-responders. None of the baseline variables discriminated responders and non-responders. However, fitting the Rohrer equation (R=K1+K2V̇) to the tracheal pressure-flow relationship over the first 0.1s of inspiration while breathing air allowed such discrimination on the basis of larger K2 in responders (0.005±.002 vs 0.002±.001 cmH2O·s2·ml-2; p<0.001), suggesting a corresponding difference in the relative contribution of laminar and turbulent flow. The differences in ΔV̇IFL between responders and non-responders were simulated by modeling the collapsible segment of the upper airways as a non-linear resistor and varying its pressure-volume curve, length and diameter, thus showing the importance of mechanical and geometrical factors in determining the response to heliox in the presence of tIFL.
Heliox administration in anesthetized rabbits with spontaneous inspiratory flow limitation / E.G. D'Angelo, M.M. Pecchiari, F. Bellemare, G. Cevenini, P. Barbini. - In: JOURNAL OF APPLIED PHYSIOLOGY. - ISSN 8750-7587. - 130:5(2021 May 11), pp. 1496-1509.
|Titolo:||Heliox administration in anesthetized rabbits with spontaneous inspiratory flow limitation|
PECCHIARI, MATTEO MARIA (Secondo) (Corresponding)
|Parole Chiave:||heliox; inspiratory flow limitation; obstructive sleep apnea and hypopnea; rabbit; upper airways|
|Settore Scientifico Disciplinare:||Settore BIO/09 - Fisiologia|
|Data di pubblicazione:||11-mag-2021|
|Data ahead of print / Data di stampa:||7-gen-2021|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1152/japplphysiol.00830.2020|
|Appare nelle tipologie:||01 - Articolo su periodico|