The A- and B-form are two of the most common structural conformations of double strand DNA present in nature and they can interchange on the basis of the helices hydration [1,2]. Herein we demonstrate that the use of non-destructive techniques such as Raman spectroscopy coupled with the use of a super-hydrophobic device, allows the clear identification of the DNA hydration state, of the backbone (phosphate + deoxyribose sugar) conformation and of the nucleotides. There is a wide prospect for an increase of knowledge in biomolecules using this combined approach resulting in a significant impact in the study of more complex supramolecular assemblies and of fine chemical variation along the genomic loci undergoing to epigenetic variations.

Raman on suspended DNA : Novel super-hydrophobic approach for structural studies / M. Marini, M. Allione, B. Torre, M. Moretti, T. Limongi, L. Tirinato, A. Giugni, G. Das, E. di Fabrizio. - In: MICROELECTRONIC ENGINEERING. - ISSN 0167-9317. - 175(2017 May 05), pp. 38-42. [10.1016/j.mee.2016.12.016]

Raman on suspended DNA : Novel super-hydrophobic approach for structural studies

A. Giugni;
2017

Abstract

The A- and B-form are two of the most common structural conformations of double strand DNA present in nature and they can interchange on the basis of the helices hydration [1,2]. Herein we demonstrate that the use of non-destructive techniques such as Raman spectroscopy coupled with the use of a super-hydrophobic device, allows the clear identification of the DNA hydration state, of the backbone (phosphate + deoxyribose sugar) conformation and of the nucleotides. There is a wide prospect for an increase of knowledge in biomolecules using this combined approach resulting in a significant impact in the study of more complex supramolecular assemblies and of fine chemical variation along the genomic loci undergoing to epigenetic variations.
A-form; B-form; DNA; Raman spectroscopy; Super-hydrophobic devices
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
24-dic-2016
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/826098
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