The shear flow generated at the rim of a drop evaporating on a micro-fabricated super-hydrophobic surface has been used to suspend and orient single/few lysozyme amyloid fibrils between two pillars for substrate-free characterization. Micro Raman spectroscopy performed on extended fibers evidenced a shift of the Amide I band main peak to the value attributed to β-sheet secondary structure, characteristic of the amyloid fibers. In addition, given the orientation sensitivity of the anisotropic molecule, the Raman signal of the main secondary structure was nicely enhanced for a fiber alignment parallel to the polarization direction of the laser. The substrate-free sample generated by this suspending technique is suitable for other structural analysis methods, where fiber crystals are investigated. It could be further employed for generation of arrays and patterns in a controllable fashion, where bio-compatible material is needed.

Raman study of lysozyme amyloid fibrils suspended on super-hydrophobic surfaces by shear flow / M. Moretti, M. Allione, M. Marini, B. Torre, A. Giugni, T. Limongi, G. Das, E. Di Fabrizio. - In: MICROELECTRONIC ENGINEERING. - ISSN 0167-9317. - 178(2017 Jun 25), pp. 194-198. [10.1016/j.mee.2017.05.045]

Raman study of lysozyme amyloid fibrils suspended on super-hydrophobic surfaces by shear flow

A. Giugni;
2017

Abstract

The shear flow generated at the rim of a drop evaporating on a micro-fabricated super-hydrophobic surface has been used to suspend and orient single/few lysozyme amyloid fibrils between two pillars for substrate-free characterization. Micro Raman spectroscopy performed on extended fibers evidenced a shift of the Amide I band main peak to the value attributed to β-sheet secondary structure, characteristic of the amyloid fibers. In addition, given the orientation sensitivity of the anisotropic molecule, the Raman signal of the main secondary structure was nicely enhanced for a fiber alignment parallel to the polarization direction of the laser. The substrate-free sample generated by this suspending technique is suitable for other structural analysis methods, where fiber crystals are investigated. It could be further employed for generation of arrays and patterns in a controllable fashion, where bio-compatible material is needed.
Amyloid fibrils; Raman spectroscopy; Super-hydrophobic micro-patterned surfaces; β-sheet secondary structure
Settore FIS/07 - Fisica Applicata(Beni Culturali, Ambientali, Biol.e Medicin)
19-mag-2017
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/825641
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