We present an algorithm, and its MATLAB implementation, based on mathematical methods to detect and localize 3D multicolor DNA FISH spots in fluorescence cell image z-stacks. This algorithm provides a method to measure the relative positioning of spots in the nucleus and inter-spot distances with the aim to enrich our understanding of the 3D spatial organization of the genome within the cell nucleus.
An Algorithm for the Analysis of the 3D Spatial Organization of the Genome / F. Gregoretti, A. Cortesi, G. Oliva, B. Bodega, L. Antonelli (METHODS IN MOLECULAR BIOLOGY). - In: Capturing Chromosome Conformation / [a cura di] B. Bodega, C. Lanzuolo. - [s.l] : Springer, 2021. - ISBN 9781071606636. - pp. 299-320 [10.1007/978-1-0716-0664-3_17]
An Algorithm for the Analysis of the 3D Spatial Organization of the Genome.
B. Bodega;
2021
Abstract
We present an algorithm, and its MATLAB implementation, based on mathematical methods to detect and localize 3D multicolor DNA FISH spots in fluorescence cell image z-stacks. This algorithm provides a method to measure the relative positioning of spots in the nucleus and inter-spot distances with the aim to enrich our understanding of the 3D spatial organization of the genome within the cell nucleus.
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