3D organization of the genome, its structural and regulatory function of cell identity, is acquiring prominent features in epigenetics studies; more efforts have been done to develop techniques that allow studying nuclear structure. Chromosome conformation capture (3C) has been set up in 2002 from Dekker and from that moment great investments were made to develop genomics variants of 3C technology (4C, 5C, Hi-C) providing new tools to investigate the shape of the genome in a more systematic and unbiased manner. 3C method allows scientists to fix dynamic and variable 3D interactions in nuclear space, and consequently to study which sequences interact, how a gene is regulated by different and distant enhancer, or how a set of enhancer could regulate transcriptional units; to follow the conformation that mediates regulation change in development; and to evaluate if this fine epigenetic mechanism is impaired in disease condition.

Chromosome conformation capture in primary human cells / A. Cortesi, B. Bodega (METHODS IN MOLECULAR BIOLOGY). - In: Polycomb group proteins : methods and protocols / [a cura di] C. Lanzuolo, B.Bodega. - New York : Humana Press, 2016. - ISBN 978-1-4939-6378-2. - pp. 213-221 [10.1007/978-1-4939-6380-5_19]

Chromosome conformation capture in primary human cells

B. Bodega
2016

Abstract

3D organization of the genome, its structural and regulatory function of cell identity, is acquiring prominent features in epigenetics studies; more efforts have been done to develop techniques that allow studying nuclear structure. Chromosome conformation capture (3C) has been set up in 2002 from Dekker and from that moment great investments were made to develop genomics variants of 3C technology (4C, 5C, Hi-C) providing new tools to investigate the shape of the genome in a more systematic and unbiased manner. 3C method allows scientists to fix dynamic and variable 3D interactions in nuclear space, and consequently to study which sequences interact, how a gene is regulated by different and distant enhancer, or how a set of enhancer could regulate transcriptional units; to follow the conformation that mediates regulation change in development; and to evaluate if this fine epigenetic mechanism is impaired in disease condition.
3D interactions; Chromosome conformation capture (3C); Nuclear structure; Primary human cells; Chromatin; Chromosomes; Genome, Human; Genomics; Humans; Nucleic Acid Conformation; Regulatory Sequences, Nucleic Acid; Epigenesis, Genetic
Settore BIO/11 - Biologia Molecolare
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/825396
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