The failure to eradicate most cancers and in particular melanoma may be as fundamental as a misidentification of the target (1). In this study, we demonstrate the presence of stem/initiating subsets in melanoma both in biopsy and in an established melanoma cell line grown in vitro and in xenografts. In particular, our data show two main novelties for human melanoma: firstly melanoma biopsy contains a subset of cells expressing CD133 and CD133+ subset cells able to develop a Mart‐1 positive tumor in NOD‐SCID mice. Secondly the WM115, a human melanoma cell line, expressed CD133 and ABCG2. This cell line grows as floating spheroids, expresses typical progenitors and mature neuronal/oligodendrocyte markers and is able to transdifferenziate into astrocytes or mesenchymal lineages under specific growth conditions. More interesting, tumor xenografts show lower levels of CD133 and ABCG2.

CD133 positive cellular population in human melanoma / C. Anna Maria la Porta, E. Monzani, F. Facchetti, E. Galmozzi, E. Corsini, A. Benetti, C. Cavazzin, A. Gritti, A.A.M. Piccinini, M. Santinami, E. Parati, G. Alessandri. - In: THE FASEB JOURNAL. - ISSN 0892-6638. - 21:5(2007 Apr), pp. 32-32. ((Intervento presentato al convegno Special Issue:Experimental Biology 2007 (Part I) [10.1096/fasebj.21.5.A32-a].

CD133 positive cellular population in human melanoma

E. Monzani;F. Facchetti;E. Galmozzi;C. Cavazzin;A.A.M. Piccinini;E. Parati;
2007

Abstract

The failure to eradicate most cancers and in particular melanoma may be as fundamental as a misidentification of the target (1). In this study, we demonstrate the presence of stem/initiating subsets in melanoma both in biopsy and in an established melanoma cell line grown in vitro and in xenografts. In particular, our data show two main novelties for human melanoma: firstly melanoma biopsy contains a subset of cells expressing CD133 and CD133+ subset cells able to develop a Mart‐1 positive tumor in NOD‐SCID mice. Secondly the WM115, a human melanoma cell line, expressed CD133 and ABCG2. This cell line grows as floating spheroids, expresses typical progenitors and mature neuronal/oligodendrocyte markers and is able to transdifferenziate into astrocytes or mesenchymal lineages under specific growth conditions. More interesting, tumor xenografts show lower levels of CD133 and ABCG2.
Settore MED/04 - Patologia Generale
Settore MED/43 - Medicina Legale
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/822608
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