The first differentiation event in mammalian embryos is the formation of the trophectoderm, which is the progenitor of the outer epithelial component of the placenta and supports the fetus during intrauterine life. Our understanding of these events is limited, particularly in human, because of ethical and legal restrictions and availability of adequate in vitro models would be very advantageous. Here we describe a method that converts human fibroblasts into trophoblast-like cells, combining the use of 5-azacytidine-CR (5-aza-CR) to erase the original cell phenotype and a cocktail containing bone morphogenetic protein 4 (BMP4) with inhibitors of the Activin/Nodal/ERK signaling pathways, to drive erased fibroblasts into the trophoblastic differentiation. This innovative method uses very easily accessible cells to derive trophoblast-like cells and it can be useful to study embryo implantation disorders related to aging.

A Two-Step Protocol to Erase Human Skin Fibroblasts and Convert Them into Trophoblast-like Cells / S. Arcuri, F. Gandolfi, E. Somigliana, T.A. Brevini (METHODS IN MOLECULAR BIOLOGY). - In: Next Generation Culture Platforms for Reliable In Vitro Models / [a cura di] T.A.L. Brevini, A. Fazeli, K. Turksen. - [s.l] : Humana, 2021. - ISBN 9781071612453. - pp. 151-158

A Two-Step Protocol to Erase Human Skin Fibroblasts and Convert Them into Trophoblast-like Cells

S. Arcuri;F. Gandolfi;E. Somigliana;T.A. Brevini
2021

Abstract

The first differentiation event in mammalian embryos is the formation of the trophectoderm, which is the progenitor of the outer epithelial component of the placenta and supports the fetus during intrauterine life. Our understanding of these events is limited, particularly in human, because of ethical and legal restrictions and availability of adequate in vitro models would be very advantageous. Here we describe a method that converts human fibroblasts into trophoblast-like cells, combining the use of 5-azacytidine-CR (5-aza-CR) to erase the original cell phenotype and a cocktail containing bone morphogenetic protein 4 (BMP4) with inhibitors of the Activin/Nodal/ERK signaling pathways, to drive erased fibroblasts into the trophoblastic differentiation. This innovative method uses very easily accessible cells to derive trophoblast-like cells and it can be useful to study embryo implantation disorders related to aging.
5-aza-CR; Activin/Nodal/ERK inhibitors; BMP4; Epigenetics; Fibroblast; Trophoblast
Settore VET/01 - Anatomia degli Animali Domestici
2021
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/819700
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