Mutations in the human X-linked cyclin-dependent kinase-like 5 (CDKL5) gene have been identified in patients with Rett syndrome (RTT), West syndrome, and X-linked infantile spasms, sharing the common feature of mental retardation and early seizures. CDKL5 is a rather uncharacterized kinase, but its involvement in RTT seems to be explained by the fact that it works upstream of MeCP2, the main cause of Rett syndrome. To understand the role of this kinase for nervous system functions and to address if molecular mechanisms are involved in regulating its distribution and activity, we studied the ontogeny of CDKL5 expression in developing mouse brains by immunostaining and Western blotting. The expression profile of CDKL5 was compared with that of MeCP2. The two proteins share a general expression profile in the adult mouse brain, but CDKL5 levels appear to be highly modulated at the regional level. Its expression is strongly induced in early postnatal stages, and in the adult brain CDKL5 is present in mature neurons, but not in astroglia. Interestingly, the presence of CDKL5 in the cell nucleus varies at the regional level of the adult brain and is developmentally regulated. CDKL5 shuttles between the cytoplasm and the nucleus and the C-terminal tail is involved in localizing the protein to the cytoplasm in a mechanism depending on active nuclear export. Accordingly, Rett derivatives containing disease-causing truncations of the C terminus are constitutively nuclear, suggesting that they might act as gain of function mutations in this cellular compartment.

CDKL5 expression is modulated during neuronal development and its subcellular distribution is tightly regulated by the C-terminal tail / L. Rusconi, L. Salvatoni, L. Giudici, I. Bertani, C. Kilstrup-Nielsen, V. Broccoli, N. Landsberger. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - 283:44(2008), pp. 30101-30111. [10.1074/jbc.M804613200]

CDKL5 expression is modulated during neuronal development and its subcellular distribution is tightly regulated by the C-terminal tail

N. Landsberger
2008

Abstract

Mutations in the human X-linked cyclin-dependent kinase-like 5 (CDKL5) gene have been identified in patients with Rett syndrome (RTT), West syndrome, and X-linked infantile spasms, sharing the common feature of mental retardation and early seizures. CDKL5 is a rather uncharacterized kinase, but its involvement in RTT seems to be explained by the fact that it works upstream of MeCP2, the main cause of Rett syndrome. To understand the role of this kinase for nervous system functions and to address if molecular mechanisms are involved in regulating its distribution and activity, we studied the ontogeny of CDKL5 expression in developing mouse brains by immunostaining and Western blotting. The expression profile of CDKL5 was compared with that of MeCP2. The two proteins share a general expression profile in the adult mouse brain, but CDKL5 levels appear to be highly modulated at the regional level. Its expression is strongly induced in early postnatal stages, and in the adult brain CDKL5 is present in mature neurons, but not in astroglia. Interestingly, the presence of CDKL5 in the cell nucleus varies at the regional level of the adult brain and is developmentally regulated. CDKL5 shuttles between the cytoplasm and the nucleus and the C-terminal tail is involved in localizing the protein to the cytoplasm in a mechanism depending on active nuclear export. Accordingly, Rett derivatives containing disease-causing truncations of the C terminus are constitutively nuclear, suggesting that they might act as gain of function mutations in this cellular compartment.
No
English
Adult; Animals; Brain; Cell Nucleus; Female; HeLa Cells; Humans; Mice; Mice, Inbred C57BL; Middle Aged; Neurons; Protein Structure, Tertiary; Protein-Serine-Threonine Kinases; Subcellular Fractions; Gene Expression Regulation; Gene Expression Regulation, Developmental
Settore BIO/11 - Biologia Molecolare
Articolo
Esperti anonimi
Pubblicazione scientifica
2008
AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
283
44
30101
30111
11
Pubblicato
Periodico con rilevanza internazionale
scopus
pubmed
crossref
wos
datacite
Aderisco
info:eu-repo/semantics/article
CDKL5 expression is modulated during neuronal development and its subcellular distribution is tightly regulated by the C-terminal tail / L. Rusconi, L. Salvatoni, L. Giudici, I. Bertani, C. Kilstrup-Nielsen, V. Broccoli, N. Landsberger. - In: THE JOURNAL OF BIOLOGICAL CHEMISTRY. - ISSN 0021-9258. - 283:44(2008), pp. 30101-30111. [10.1074/jbc.M804613200]
open
Prodotti della ricerca::01 - Articolo su periodico
7
262
Article (author)
no
L. Rusconi, L. Salvatoni, L. Giudici, I. Bertani, C. Kilstrup-Nielsen, V. Broccoli, N. Landsberger
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/816769
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