To elucidate the role of sialomucin in friction reduction, we investigated the sliding friction of pleural mesothelial cells monolayers cultured on fibrine gel. These measurements were performed on normal (4/4 RM-4) and on tumor (CARM-L1 TG3) cell lines. The effect of treatment with neuraminidase, which removes sialic acid from sialomucin, and of dexamethasone, which has shown to increase sialomucin expression, were also assessed. Furthermore, the expression of the main form of cell-surface-associated mucin (MUC1) present in the mesothelium, was assessed by western blot and immunofluorescence, under different experimental conditions. Expression of MUC1 was not significantly different in the two cell lines. Moreover, dexamethasone did not increase the expression of MUC1. Coefficient of kinetic friction (μ) was significantly higher in tumor cells than in normal cells. Neuraminidase increased μ in both cell lines. These results suggest that sialomucin may play a role in reducing the friction of pleural mesothelial cells.

Role of MUC1 in lubrication of pleural mesothelialcells cultured on fibrine gel / F. Bodega, C.M.E. Sironi, L.A.M. Zocchi, C.E.H. Porta. - In: TISSUE & CELL. - ISSN 0040-8166. - (2021). [Epub ahead of print]

Role of MUC1 in lubrication of pleural mesothelialcells cultured on fibrine gel

F. Bodega
Primo
;
C.M.E. Sironi
Secondo
;
L.A.M. Zocchi
Penultimo
;
C.E.H. Porta
Ultimo
2021

Abstract

To elucidate the role of sialomucin in friction reduction, we investigated the sliding friction of pleural mesothelial cells monolayers cultured on fibrine gel. These measurements were performed on normal (4/4 RM-4) and on tumor (CARM-L1 TG3) cell lines. The effect of treatment with neuraminidase, which removes sialic acid from sialomucin, and of dexamethasone, which has shown to increase sialomucin expression, were also assessed. Furthermore, the expression of the main form of cell-surface-associated mucin (MUC1) present in the mesothelium, was assessed by western blot and immunofluorescence, under different experimental conditions. Expression of MUC1 was not significantly different in the two cell lines. Moreover, dexamethasone did not increase the expression of MUC1. Coefficient of kinetic friction (μ) was significantly higher in tumor cells than in normal cells. Neuraminidase increased μ in both cell lines. These results suggest that sialomucin may play a role in reducing the friction of pleural mesothelial cells.
pleura; friction; MUC1; fibrine gel; mesothelial cells; neuraminidase; dexamethasone;
Settore BIO/09 - Fisiologia
29-gen-2021
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/811029
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