Oral carcinoma is the most common of malignant tumors of the cavity oral and is still a serious problem for human health with an impact clinical in terms of incidence, prevalence and mortality rates that does not tend to improve. Diagnosis is often made when the malignancy is advanced and consequently the prognosis is poor with high morbidity and mortality. High-risk human papilloma viruses (HPV) types 16 and 18 as aetiological agents of anogenital carcinoma have been firmly established in the literature. Because of the morphological similarities and epitheliotropic nature of HPV, a link between OSSC and HPV seemed logical and has been the focus of numerous studies. The relationship between HPV and the oral mucosa has been supported by several investigators reporting the presence of HPV-DNA in healthy oral mucosa. During the first year of attendance at the Pathology and Oral Medicine Outpatient Clinic of the Cà Granda IRCCS Hospital in Milan, the design bases were laid for a search for HPV serotypes particularly related to the possible predisposition and onset of oral lesion muco-cutaneous and oral and oro-pharyngeal neoplasms. The second part of the study contemplate the recruitment of subjects investigated through biopsy and histopathological examination for the diagnosis of HPV pathologies related to the mucous membranes of the oral cavity (squamous papilloma, acute condyloma, verruca vulgaris) attending Pathology and Oral Medicine Outpatient Clinic of the Ca’Granda IRCCS Hospital in Milan. 12-month recruitment period from October 2018 to October 2019. The intent of this initial study, which will progress beyond the conclusion of this path, is to evaluate a rapid, effective and achievable method on a large scale to extend the research of HPV even in patients not suffering from overt correctable HPV pathologies in order to plan a screening for the follow-up of the development of related HPV oral cancer genotyping in the study of cancer is useful for providing further data on the causal relationship between cancer and viral infection, on oncogenic HPVs and on the cellular modifications they induce. On the vaccinated population, it allows to monitor the duration and effectiveness of protection and is useful in stratifying HPV positive women and men based on the risk of developing precancerous or cancerous lesions and thus to guide clinical decisions. The genotyping tests in use today differ in their analytical capabilities for sensitivity and specific type specificity. In the vaccination era, a high analytical sensitivity is required, as a failure to identify the prevalent infections upon entry into a trial can lead to a false result of vaccine failure in vaccination protocols.

HPV NEL DISTRETTO TESTA COLLO:SCREENING MOLECOLARE PER UNA VALUTAZIONE EPIDEMIOLOGICA SU CAMPIONI ITALIANI / V. Candotto ; supervisori della tesi: F. CARINCI, L. LO MUZIO, F. SPADARI ; coordinatore: M. DEL FABBRO. - : . Dipartimento di Scienze Biomediche, Chirurgiche ed Odontoiatriche, 2021 Mar 01. ((33. ciclo, Anno Accademico 2020. [10.13130/candotto-valentina_phd2021-03-01].

HPV NEL DISTRETTO TESTA COLLO:SCREENING MOLECOLARE PER UNA VALUTAZIONE EPIDEMIOLOGICA SU CAMPIONI ITALIANI

V. Candotto
2021-03-01

Abstract

Oral carcinoma is the most common of malignant tumors of the cavity oral and is still a serious problem for human health with an impact clinical in terms of incidence, prevalence and mortality rates that does not tend to improve. Diagnosis is often made when the malignancy is advanced and consequently the prognosis is poor with high morbidity and mortality. High-risk human papilloma viruses (HPV) types 16 and 18 as aetiological agents of anogenital carcinoma have been firmly established in the literature. Because of the morphological similarities and epitheliotropic nature of HPV, a link between OSSC and HPV seemed logical and has been the focus of numerous studies. The relationship between HPV and the oral mucosa has been supported by several investigators reporting the presence of HPV-DNA in healthy oral mucosa. During the first year of attendance at the Pathology and Oral Medicine Outpatient Clinic of the Cà Granda IRCCS Hospital in Milan, the design bases were laid for a search for HPV serotypes particularly related to the possible predisposition and onset of oral lesion muco-cutaneous and oral and oro-pharyngeal neoplasms. The second part of the study contemplate the recruitment of subjects investigated through biopsy and histopathological examination for the diagnosis of HPV pathologies related to the mucous membranes of the oral cavity (squamous papilloma, acute condyloma, verruca vulgaris) attending Pathology and Oral Medicine Outpatient Clinic of the Ca’Granda IRCCS Hospital in Milan. 12-month recruitment period from October 2018 to October 2019. The intent of this initial study, which will progress beyond the conclusion of this path, is to evaluate a rapid, effective and achievable method on a large scale to extend the research of HPV even in patients not suffering from overt correctable HPV pathologies in order to plan a screening for the follow-up of the development of related HPV oral cancer genotyping in the study of cancer is useful for providing further data on the causal relationship between cancer and viral infection, on oncogenic HPVs and on the cellular modifications they induce. On the vaccinated population, it allows to monitor the duration and effectiveness of protection and is useful in stratifying HPV positive women and men based on the risk of developing precancerous or cancerous lesions and thus to guide clinical decisions. The genotyping tests in use today differ in their analytical capabilities for sensitivity and specific type specificity. In the vaccination era, a high analytical sensitivity is required, as a failure to identify the prevalent infections upon entry into a trial can lead to a false result of vaccine failure in vaccination protocols.
SPADARI, FRANCESCO
DEL FABBRO, MASSIMO
SPADARI, FRANCESCO
Settore MED/28 - Malattie Odontostomatologiche
HPV NEL DISTRETTO TESTA COLLO:SCREENING MOLECOLARE PER UNA VALUTAZIONE EPIDEMIOLOGICA SU CAMPIONI ITALIANI / V. Candotto ; supervisori della tesi: F. CARINCI, L. LO MUZIO, F. SPADARI ; coordinatore: M. DEL FABBRO. - : . Dipartimento di Scienze Biomediche, Chirurgiche ed Odontoiatriche, 2021 Mar 01. ((33. ciclo, Anno Accademico 2020. [10.13130/candotto-valentina_phd2021-03-01].
Doctoral Thesis
File in questo prodotto:
File Dimensione Formato  
phd_unimi_R11849.pdf

accesso aperto

Tipologia: Tesi di dottorato completa
Dimensione 592.61 kB
Formato Adobe PDF
592.61 kB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/804101
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact