We present a method to modify bacterial artificial chromosomes (BACs) resident in their host strain. The method is based on homologous recombination by ET-cloning. We have successfully modified BACs at two distinct loci by recombination with a PCR product containing homology arms of 50 nt. The procedure we describe here is rapid, was found to work with high efficiency and should be applicable to any BAC modification desired.

Rapid modification of bacterial artificial chromosomes by ET-recombination / J.P.P. Muyrers, Y. Zhang, G. Testa, A.F. Stewart. - In: NUCLEIC ACIDS RESEARCH. - ISSN 0305-1048. - 27:6(1999), pp. 1555-1557. [10.1093/nar/27.6.1555]

Rapid modification of bacterial artificial chromosomes by ET-recombination

G. Testa;
1999

Abstract

We present a method to modify bacterial artificial chromosomes (BACs) resident in their host strain. The method is based on homologous recombination by ET-cloning. We have successfully modified BACs at two distinct loci by recombination with a PCR product containing homology arms of 50 nt. The procedure we describe here is rapid, was found to work with high efficiency and should be applicable to any BAC modification desired.
cloning
Settore BIO/11 - Biologia Molecolare
Settore BIO/13 - Biologia Applicata
Settore BIO/18 - Genetica
Settore MED/03 - Genetica Medica
Settore BIO/10 - Biochimica
1999
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Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/802231
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