In recent years, protein science has been revolutionized by the discovery of intrinsically disordered proteins (IDPs). In contrast to the classical paradigm that a given protein sequence corresponds to a defined structure and an associated function, we now know that proteins can be functional in the absence of a stable three-dimensional structure. In many cases, disordered proteins or protein regions become structured, at least locally, upon interacting with their physiological partners. Many, sometimes conflicting, hypotheses have been put forward regarding the interaction mechanisms of IDPs and the potential advantages of disorder for protein-protein interactions. Whether disorder may increase, as proposed, e.g., in the "fly-casting" hypothesis, or decrease binding rates, increase or decrease binding specificity, or what role pre-formed structure might play in interactions involving IDPs (conformational selection vs. induced fit), are subjects of intense debate. Experimentally, these questions remain difficult to address. Here, we review experimental studies of binding mechanisms of IDPs using NMR spectroscopy and transient kinetic techniques, as well as the underlying theoretical concepts and numerical methods that can be applied to describe these interactions at the atomic level. The available literature suggests that the kinetic and thermodynamic parameters characterizing interactions involving IDPs can vary widely and that there may be no single common mechanism that can explain the different binding modes observed experimentally. Rather, disordered proteins appear to make combined use of features such as pre-formed structure and flexibility, depending on the individual system and the functional context.
Binding mechanisms of intrinsically disordered proteins : theory, simulation, and experiment / L. Mollica, L.M. Bessa, X. Hanoulle, M.R. Jensen, M. Blackledge, R. Schneider. - In: FRONTIERS IN MOLECULAR BIOSCIENCES. - ISSN 2296-889X. - 3(2016 Sep), pp. 52.1-52.18.
|Titolo:||Binding mechanisms of intrinsically disordered proteins : theory, simulation, and experiment|
|Parole Chiave:||Intrinsically disordered proteins; Kinetics; Molecular dynamics simulations; Nuclear magnetic resonance; Protein-protein interactions|
|Settore Scientifico Disciplinare:||Settore CHIM/02 - Chimica Fisica|
Settore BIO/10 - Biochimica
|Data di pubblicazione:||set-2016|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3389/fmolb.2016.00052|
|Appare nelle tipologie:||01 - Articolo su periodico|