The microbiome is now seen as an important resource to understand animal health and welfare in many species. However, there are few studies aiming at identifying the association between fecal microbiome composition and husbandry conditions in sheep. A wide range of stressors associated with management and housing of animals increases the hypothalamic–pituitary axis activity, with growing evidence that the microbiome composition can be modified. Therefore, the purpose of the present study was to describe the core microbiome in sheep, characterized using 16S rRNA gene sequencing, and to explore whether exposure to stressful husbandry conditions changed sheep hindgut microbiome composition. Sheep (n = 10) were divided in two groups: isolated group (individually separated for 3 h/day) and control group (housed in the home pen for the entire trial period). Sheep core microbiome was dominated by Firmicutes (43.6%), Bacteroidetes (30.38%), Proteobacteria (10.14%), and Verrucomicrobia (7.55%). Comparative results revealed few operational taxonomic units (OTUs) with significantly different relative abundance between groups. Chao1, abundance-based coverage estimator (ACE), and Fisher’s alpha indices did not show differences between groups. OTU-based Bray–Curtis distances between groups were not significant (p-value = 0.07). In conclusion, these results describing the core microbiome of sheep do not suggest a strong effect of stressful husbandry conditions on microbial composition.

Analysis of hindgut microbiome of sheep and effect of different husbandry conditions / G. Minozzi, F. Biscarini, E. Dalla Costa, M. Chincarini, N. Ferri, C. Palestrini, M. Minero, S. Mazzola, R. Piccinini, G. Vignola, S. Cannas. - In: ANIMALS. - ISSN 2076-2615. - 11:1(2021 Jan 11), pp. 4.1-4.14. [10.3390/ani11010004]

Analysis of hindgut microbiome of sheep and effect of different husbandry conditions

G. Minozzi
Primo
;
E. Dalla Costa;N. Ferri;C. Palestrini;M. Minero;S. Mazzola;R. Piccinini;S. Cannas
Ultimo
2021-01-11

Abstract

The microbiome is now seen as an important resource to understand animal health and welfare in many species. However, there are few studies aiming at identifying the association between fecal microbiome composition and husbandry conditions in sheep. A wide range of stressors associated with management and housing of animals increases the hypothalamic–pituitary axis activity, with growing evidence that the microbiome composition can be modified. Therefore, the purpose of the present study was to describe the core microbiome in sheep, characterized using 16S rRNA gene sequencing, and to explore whether exposure to stressful husbandry conditions changed sheep hindgut microbiome composition. Sheep (n = 10) were divided in two groups: isolated group (individually separated for 3 h/day) and control group (housed in the home pen for the entire trial period). Sheep core microbiome was dominated by Firmicutes (43.6%), Bacteroidetes (30.38%), Proteobacteria (10.14%), and Verrucomicrobia (7.55%). Comparative results revealed few operational taxonomic units (OTUs) with significantly different relative abundance between groups. Chao1, abundance-based coverage estimator (ACE), and Fisher’s alpha indices did not show differences between groups. OTU-based Bray–Curtis distances between groups were not significant (p-value = 0.07). In conclusion, these results describing the core microbiome of sheep do not suggest a strong effect of stressful husbandry conditions on microbial composition.
sheep; hindgut microbiome; husbandry conditions; physiology; hair cortisol
Settore AGR/19 - Zootecnica Speciale
approach to the investigation of cerebral activity in domestic animals using functional near-infrared spectroscopy: Implications on animal welfare and on comparative system and cognitive neuroscience.
Genomica e Sostenibilità in Apicoltura (BEENOMIX 2.0)
23-dic-2020
Article (author)
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Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/800697
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