The interactions taking place between a dental (bio)material, the surrounding tissues of the host, and the biofilm that grows to permanently colonize this microenvironment are amazingly complex when analyzed in detail yet contribute to a crucial factor: the balance between health and disease conditions. From a microbiological point of view, this has a dramatic impact on the longevity of dental treatments. Researchers have long since tried to recreate, even if in parts, this complexity on a bench, both using a reductionistic approach as often performed in research and, more recently, by trying to create models approaching the most realistic behavior. These efforts yielded a wide range of bioreactor systems currently available. We hope that in a future not too far, bioreactor models will be able to reliably reproduce most clinical conditions, dramatically reducing the need for animal and clinical studies. Unfortunately, a universal bioreactor able to mimic any clinical situation still does not exist. Each model comes entwined with its advantages and limitations that must be acknowledged when choosing which model best fits a distinct experimental design. This situation, together with a reduced overall level of standardization, makes the comparison of the obtained results very difficult. This chapter presents an overview of the microbial communities and the bioreactor models that are most significant for studying the microbiological performances of dental materials.

Bioreactors: How to Study Biofilms In Vitro / A.C. Ionescu, E. Brambilla - In: Oral Biofilms and Modern Dental Materials : Advances Toward Bioactivity / [a cura di] A.C. Ionescu, S. Hahnel. - Prima edizione. - [s.l] : Springer Nature, 2021. - ISBN 9783030673871. - pp. 37-54 [10.1007/978-3-030-67388-8_4]

Bioreactors: How to Study Biofilms In Vitro

A.C. Ionescu;E. Brambilla
2021

Abstract

The interactions taking place between a dental (bio)material, the surrounding tissues of the host, and the biofilm that grows to permanently colonize this microenvironment are amazingly complex when analyzed in detail yet contribute to a crucial factor: the balance between health and disease conditions. From a microbiological point of view, this has a dramatic impact on the longevity of dental treatments. Researchers have long since tried to recreate, even if in parts, this complexity on a bench, both using a reductionistic approach as often performed in research and, more recently, by trying to create models approaching the most realistic behavior. These efforts yielded a wide range of bioreactor systems currently available. We hope that in a future not too far, bioreactor models will be able to reliably reproduce most clinical conditions, dramatically reducing the need for animal and clinical studies. Unfortunately, a universal bioreactor able to mimic any clinical situation still does not exist. Each model comes entwined with its advantages and limitations that must be acknowledged when choosing which model best fits a distinct experimental design. This situation, together with a reduced overall level of standardization, makes the comparison of the obtained results very difficult. This chapter presents an overview of the microbial communities and the bioreactor models that are most significant for studying the microbiological performances of dental materials.
Bioreactors; Microfluidics; Biofilms; Oral microcosm; Defined consortium; Streptococcus mutans; Standardization
Settore MED/28 - Malattie Odontostomatologiche
Book Part (author)
File in questo prodotto:
File Dimensione Formato  
Chapter 3 Bioreactors how to study biofilms in vitro.pdf

accesso riservato

Tipologia: Pre-print (manoscritto inviato all'editore)
Dimensione 648.99 kB
Formato Adobe PDF
648.99 kB Adobe PDF   Visualizza/Apri   Richiedi una copia
Ionescu-Brambilla2021_Chapter_BioreactorsHowToStudyBiofilmsI.pdf

accesso riservato

Tipologia: Publisher's version/PDF
Dimensione 1.99 MB
Formato Adobe PDF
1.99 MB Adobe PDF   Visualizza/Apri   Richiedi una copia
Pubblicazioni consigliate

Caricamento pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: http://hdl.handle.net/2434/800419
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus ND
  • ???jsp.display-item.citation.isi??? ND
social impact