The environment, including animals and animal products, is colonized by bacterial species that are typical and specific of every different ecological niche. Natural and human-related ecological pressure promotes the selection and expression of genes related to antimicrobial resistance (AMR). These genes might be present in a bacterial consortium but might not necessarily be expressed. Their expression could be induced by the presence of antimicrobial compounds that could originate from a given ecological niche or from human activity. In this work, we applied (meta)proteomics analysis of bacterial compartment of raw milk in order to obtain a method that provides a measurement of circulating AMR involved proteins and gathers information about the whole bacterial composition. Results from milk analysis revealed the presence of 29 proteins/proteoforms linked to AMR. The detection of mainly β-lactamases suggests the possibility of using the milk microbiome as a bioindicator for the investigation of AMR. Moreover, it was possible to achieve a culture-free qualitative and functional analysis of raw milk bacterial consortia.
Raw cow milk bacterial consortium as bioindicator of circulating anti-microbial resistance (AMR) / C. Piras, V. Greco, E. Gugliandolo, A. Soggiu, B. Tilocca, L. Bonizzi, A. Zecconi, R. Cramer, D. Britti, A. Urbani, P. Roncada. - In: ANIMALS. - ISSN 2076-2615. - 10:12(2020 Dec 11), pp. 2378.1-2378.13.
|Titolo:||Raw cow milk bacterial consortium as bioindicator of circulating anti-microbial resistance (AMR)|
|Parole Chiave:||proteomics; AMR; raw milk; microbiome; β-lactamase|
|Settore Scientifico Disciplinare:||Settore VET/05 - Malattie Infettive degli Animali Domestici|
|Progetto:||Safe milk: omics science for milk safety and quality (SAFE MILK)|
|Data di pubblicazione:||11-dic-2020|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3390/ani10122378|
|Appare nelle tipologie:||01 - Articolo su periodico|