Mechanotransduction is the ability of cells to translate mechanical stimuli into biochemical signals that can ultimately influence gene expression, cell morphology and cell fate. Tenocytes are responsible for tendon mechanical adaptation converting mechanical stimuli imposed during mechanical loading, thus affecting extracellular matrix homeostasis. Since we previously demonstrated that MD-Tissue, an injectable collagen-based medical compound containing swine-derived collagen as the main component, is able to affect tenocyte properties, the aim of this study was to analyze whether the effects triggered by MD-Tissue were based on mechanotransduction-related mechanisms. For this purpose, MD-Tissue was used to coat Petri dishes and cytochalasin B was used to deprive tenocytes of mechanical stimulation mediated by the actin cytoskeleton. Cell morphology, migration, collagen turnover pathways and the expression of key mechanosensors were analyzed by morphological and molecular methods. Our findings confirm that MD-Tissue affects collagen turnover pathways and favors cell migration and show that the MD-Tissue-induced effect represents a mechanical input involving the mechanotransduction machinery. Overall, MD-Tissue, acting as a mechanical scaffold, could represent an effective medical device for a novel therapeutic, regenerative and rehabilitative approach to favor tendon healing in tendinopathies.
The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes / F. Randelli, P. Sartori, C. Carlomagno, M. Bedoni, A. Menon, E. Vezzoli, M. Sommariva, N. Gagliano. - In: CELLS. - ISSN 2073-4409. - 9:12(2020 Dec), pp. 2641.1-2641.20.
|Titolo:||The Collagen-Based Medical Device MD-Tissue Acts as a Mechanical Scaffold Influencing Morpho-Functional Properties of Cultured Human Tenocytes|
GAGLIANO, NICOLETTA (Ultimo) (Corresponding)
|Parole Chiave:||tendon; tenocytes; tendinopathy; collagen turnover; mechanotransduction; actin cytoskeleton; YAP/TAZ; medical device|
|Settore Scientifico Disciplinare:||Settore BIO/16 - Anatomia Umana|
Settore BIO/17 - Istologia
|Data di pubblicazione:||dic-2020|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.3390/cells9122641|
|Appare nelle tipologie:||01 - Articolo su periodico|