The mechanisms for the regulation of homeotic genes are poorly understood in most organisms, including plants. We identified BASIC PENTACYSTEINE1 (BPC1) as a regulator of the homeotic Arabidopsis thaliana gene SEEDSTICK (STK), which controls ovule identity, and characterized its mechanism of action. A combination of tethered particle motion analysis and electromobility shift assays revealed that BPC1 is able to induce conformational changes by cooperative binding to purine-rich elements present in the STK regulatory sequence. Analysis of STK expression in the bpc1 mutant showed that STK is upregulated. Our results give insight into the regulation of gene expression in plants and provide the basis for further studies to understand the mechanisms that control ovule identity in Arabidopsis.

Basic Pentacysteine1, a GA binding protein that induces conformational changes in the regulatory region of the homeotic Arabidopsis gene Seedstick / M. Kooiker, C.A. Airoldi, A. Losa, P.S. Manzotti, L. Finzi, M.M. Kater, L. Colombo. - In: PLANT CELL. - ISSN 1040-4651. - 17:3(2005 Mar), pp. 722-729.

Basic Pentacysteine1, a GA binding protein that induces conformational changes in the regulatory region of the homeotic Arabidopsis gene Seedstick

M. Kooiker
Primo
;
C.A. Airoldi
Secondo
;
A. Losa;P.S. Manzotti;L. Finzi;M.M. Kater
Penultimo
;
L. Colombo
Ultimo
2005

Abstract

The mechanisms for the regulation of homeotic genes are poorly understood in most organisms, including plants. We identified BASIC PENTACYSTEINE1 (BPC1) as a regulator of the homeotic Arabidopsis thaliana gene SEEDSTICK (STK), which controls ovule identity, and characterized its mechanism of action. A combination of tethered particle motion analysis and electromobility shift assays revealed that BPC1 is able to induce conformational changes by cooperative binding to purine-rich elements present in the STK regulatory sequence. Analysis of STK expression in the bpc1 mutant showed that STK is upregulated. Our results give insight into the regulation of gene expression in plants and provide the basis for further studies to understand the mechanisms that control ovule identity in Arabidopsis.
binding proteins ; gene expression ; genes ; genetic regulation ; mutants ; ovules ; purines
Settore BIO/01 - Botanica Generale
Settore BIO/04 - Fisiologia Vegetale
Settore BIO/18 - Genetica
mar-2005
Article (author)
File in questo prodotto:
File Dimensione Formato  
722.full.pdf

accesso aperto

Tipologia: Publisher's version/PDF
Dimensione 310.57 kB
Formato Adobe PDF
310.57 kB Adobe PDF Visualizza/Apri
Pubblicazioni consigliate

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/2434/7951
Citazioni
  • ???jsp.display-item.citation.pmc??? 48
  • Scopus 102
  • ???jsp.display-item.citation.isi??? 97
social impact