The mechanisms for the regulation of homeotic genes are poorly understood in most organisms, including plants. We identified BASIC PENTACYSTEINE1 (BPC1) as a regulator of the homeotic Arabidopsis thaliana gene SEEDSTICK (STK), which controls ovule identity, and characterized its mechanism of action. A combination of tethered particle motion analysis and electromobility shift assays revealed that BPC1 is able to induce conformational changes by cooperative binding to purine-rich elements present in the STK regulatory sequence. Analysis of STK expression in the bpc1 mutant showed that STK is upregulated. Our results give insight into the regulation of gene expression in plants and provide the basis for further studies to understand the mechanisms that control ovule identity in Arabidopsis.
Basic Pentacysteine1, a GA binding protein that induces conformational changes in the regulatory region of the homeotic Arabidopsis gene Seedstick / M. Kooiker, C.A. Airoldi, A. Losa, P.S. Manzotti, L. Finzi, M.M. Kater, L. Colombo. - In: PLANT CELL. - ISSN 1040-4651. - 17:3(2005 Mar), pp. 722-729.
|Titolo:||Basic Pentacysteine1, a GA binding protein that induces conformational changes in the regulatory region of the homeotic Arabidopsis gene Seedstick|
KOOIKER, MAARTEN (Primo)
AIROLDI, CHIARA (Secondo)
KATER, MARTIN (Penultimo)
COLOMBO, LUCIA (Ultimo)
|Parole Chiave:||binding proteins ; gene expression ; genes ; genetic regulation ; mutants ; ovules ; purines|
|Settore Scientifico Disciplinare:||Settore BIO/01 - Botanica Generale|
Settore BIO/04 - Fisiologia Vegetale
Settore BIO/18 - Genetica
|Data di pubblicazione:||mar-2005|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1105/tpc.104.030130|
|Appare nelle tipologie:||01 - Articolo su periodico|