The mechanistic target of rapamycin complex 1 (mTORC1) is a key metabolic hub that controls the cellular response to environmental cues by exerting its kinase activity on multiple substrates1–3. However, whether mTORC1 responds to diverse stimuli by differentially phosphorylating specific substrates is poorly understood. Here we show that transcription factor EB (TFEB), a master regulator of lysosomal biogenesis and autophagy4,5, is phosphorylated by mTORC1 via a substrate-specific mechanism that is mediated by Rag GTPases. Owing to this mechanism, the phosphorylation of TFEB—unlike other substrates of mTORC1, such as S6K and 4E-BP1— is strictly dependent on the amino-acid-mediated activation of RagC and RagD GTPases, but is insensitive to RHEB activity induced by growth factors. This mechanism has a crucial role in Birt–Hogg–Dubé syndrome, a disorder that is caused by mutations in the RagC and RagD activator folliculin (FLCN) and is characterized by benign skin tumours, lung and kidney cysts and renal cell carcinoma6,7. We found that constitutive activation of TFEB is the main driver of the kidney abnormalities and mTORC1 hyperactivity in a mouse model of Birt–Hogg–Dubé syndrome. Accordingly, depletion of TFEB in kidneys of these mice fully rescued the disease phenotype and associated lethality, and normalized mTORC1 activity. Our findings identify a mechanism that enables differential phosphorylation of mTORC1 substrates, the dysregulation of which leads to kidney cysts and cancer.
A substrate-specific mTORC1 pathway underlies Birt–Hogg–Dubé syndrome / G. Napolitano, C. Di Malta, A. Esposito, M.E.G. de Araujo, S. Pece, G. Bertalot, M. Matarese, V. Benedetti, A. Zampelli, T. Stasyk, D. Siciliano, A. Venuta, M. Cesana, C. Vilardo, E. Nusco, J. Monfregola, A. Calcagni, P.P. Di Fiore, L.A. Huber, A. Ballabio. - In: NATURE. - ISSN 0028-0836. - 585:7826(2020 Oct), pp. 597-602.
|Titolo:||A substrate-specific mTORC1 pathway underlies Birt–Hogg–Dubé syndrome|
|Parole Chiave:||Animals; Basic Helix-Loop-Helix Leucine Zipper Transcription Factors; Birt-Hogg-Dube Syndrome; Cell Line; Disease Models, Animal; Enzyme Activation; HeLa Cells; Humans; Kidney Neoplasms; Mechanistic Target of Rapamycin Complex 1; Mice; Mice, Knockout; Monomeric GTP-Binding Proteins; Phosphorylation; Protein Binding; Proto-Oncogene Proteins; Ras Homolog Enriched in Brain Protein; Substrate Specificity; Tuberous Sclerosis Complex 2 Protein; Tumor Suppressor Proteins|
|Settore Scientifico Disciplinare:||Settore MED/04 - Patologia Generale|
|Data di pubblicazione:||ott-2020|
|Digital Object Identifier (DOI):||http://dx.doi.org/10.1038/s41586-020-2444-0|
|Appare nelle tipologie:||01 - Articolo su periodico|